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Article Dans Une Revue (Compte-Rendu De Lecture) Methods in Molecular Biology Année : 2022

Lineage Tracing Methods to Study Mammary Epithelial Hierarchies In Vivo

Verónica Rodilla

Résumé

Lineage tracing is now considered the gold standard approach to study cellular hierarchies and cell fate in vivo (McKenna and Gagnon, Development 146:dev169730, 2019; Kretzschmar and Watt, Cell 148:33-45, 2012). This type of clonal analysis consists of genetically labeling defined cells and following their destiny and progeny in vivo and in situ.Here we will describe different existing in vivo systems to clonally trace targeted cells and will discuss their respective advantages and inconveniences; we will then provide stepwise instructions for setting up and evaluate lineage tracing experiments, listing the most common downstream analyses and read-out assays.
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Dates et versions

hal-03941397 , version 1 (16-01-2023)

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Verónica Rodilla, Silvia Fre. Lineage Tracing Methods to Study Mammary Epithelial Hierarchies In Vivo. Methods in Molecular Biology, 2022, Methods in Molecular Biology, 2471, pp.141-157. ⟨10.1007/978-1-0716-2193-6_7⟩. ⟨hal-03941397⟩
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