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Article Dans Une Revue ACS Nano Année : 2015

Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors

Résumé

Podosomes are mechanosensitive adhesion cell structures that are capable of applying protrusive forces onto the extracellular environment. We have recently developed a method dedicated to the evaluation of the nanoscale forces that podosomes generate to protrude into the extracellular matrix. It consists in measuring by atomic force microscopy (AFM) the nanometer deformations produced by macrophages on a compliant Formvar membrane and has been called protrusion force microscopy (PFM). Here we perform time-lapse PFM experiments and investigate spatial correlations of force dynamics between podosome pairs. We use an automated procedure based on finite element simulations that extends the analysis of PFM experimental data to take into account podosome architecture and organization. We show that protrusion force varies in a synchronous manner for podosome first neighbors, a result that correlates with phase synchrony of core F-actin temporal oscillations. This dynamic spatial coordination between podosomes suggests a short-range interaction that regulates their mechanical activity.
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Dates et versions

hal-01767608 , version 1 (16-04-2018)

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Amsha Proag, Anais Bouissou, Thomas Mangeat, Raphael Voituriez, Patrick Delobelle, et al.. Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors. ACS Nano, 2015, 9 (4), pp.3800 - 3813. ⟨10.1021/nn506745r⟩. ⟨hal-01767608⟩
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