Measure and characterization of the forces exerted by growing multicellular spheroids using microdevice arrays - LAAS-Micro Nano Bio Technologies Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2019

Measure and characterization of the forces exerted by growing multicellular spheroids using microdevice arrays

Résumé

Growing multicellular spheroids recapitulate many features of expanding microtumours, and therefore they are an attractive system for biomechanical studies. Here, we report an original approach to measure and characterize the forces exerted by proliferating multicellular spheroids. As force sensors, we used high aspect ratio PDMS pillars arranged as a ring that supports a growing breast tumour cell spheroid. After optical imaging and determination of the force application zones, we combined 3D reconstruction of the shape of each deformed PDMS pillar with the finite element method to extract the forces responsible for the experimental observation. We found that the force exerted by growing spheroids ranges between 100nN and 300nN. Moreover, the exerted force was dependent on the pillar stiffness and increased over time with spheroid growth.
Fichier principal
Vignette du fichier
journal.pone.0217227.pdf (2.06 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-02361234 , version 1 (13-11-2019)

Identifiants

Citer

Laurene Aoun, Stanislas Larnier, Pierre Weiss, Martine Cazales, Ariane Herbulot, et al.. Measure and characterization of the forces exerted by growing multicellular spheroids using microdevice arrays. PLoS ONE, 2019, 14 (5), pp.e0217227. ⟨10.1371/journal.pone.0217227⟩. ⟨hal-02361234⟩
80 Consultations
85 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More