Laser induced post-desolvation of MALDI clusters - Analyse, Interactions Moléculaires et Cellulaires Accéder directement au contenu
Article Dans Une Revue International Journal of Mass Spectrometry Année : 2016

Laser induced post-desolvation of MALDI clusters

Résumé

Despite being controversial for many years, it is now widely accepted that the Matrix Assisted Laser Desorption/Ionization (MALDI) process leads to the ejection of aggregates or clusters in addition to isolated particles and ions. Clusters composed of analyte surrounded by matrix molecules exhibit a distribution in size and are relatively stable. Several studies have demonstrated that these clusters do not necessarily fully desolvate before ion extraction, but rather progressively along the ion path. This leads to a decrease in analytical performances since these non-desolvated clusters do not contribute to the ion signal but also generate an important chemical background noise. Therefore, proper cluster desolvation before ion extraction in order to release naked analyte ions in the gas phase holds great promise of improved mass spectra sensitivity. This work presents a set-up allowing cluster desolvation using nanosecond pulses of a near-infrared laser that intercepts the expanding MALDI plume before ion extraction.
Fichier principal
Vignette du fichier
Diologent_Laser_Induced.pdf (1004.24 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01420190 , version 1 (20-12-2016)

Identifiants

Citer

Laurent Diologent, Gérard Bolbach, Cristian Focsa, Michael Ziskind, Isabelle Fournier. Laser induced post-desolvation of MALDI clusters. International Journal of Mass Spectrometry, 2016, 416, pp.29-36. ⟨10.1016/j.ijms.2016.12.005⟩. ⟨hal-01420190⟩
227 Consultations
196 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More