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            <title xml:lang="en">STRUCTURAL AND DYNAMIC STUDY BY NUCLEAR MAGNETIC RESONANCE OF A LIPID TRANSFERT PROTEIN ISOLATED IN TOBACCO</title>
            <title xml:lang="fr">ÉTUDE STRUCTURALE ET DYNAMIQUE PAR RÉSONANCE MAGNÉTIQUE NUCLÉAIRE, D'UNE PROTÉINE DE TRANSFERT DE LIPIDES ISOLÉE DANS LE TABAC</title>
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                <title xml:lang="en">STRUCTURAL AND DYNAMIC STUDY BY NUCLEAR MAGNETIC RESONANCE OF A LIPID TRANSFERT PROTEIN ISOLATED IN TOBACCO</title>
                <title xml:lang="fr">ÉTUDE STRUCTURALE ET DYNAMIQUE PAR RÉSONANCE MAGNÉTIQUE NUCLÉAIRE, D'UNE PROTÉINE DE TRANSFERT DE LIPIDES ISOLÉE DANS LE TABAC</title>
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                <authority type="jury">Vovelle Françoise (Directeur de thèse)</authority>
                <authority type="jury">Delpierre Muriel (Rapporteur)</authority>
                <authority type="jury">Simorre Jean-Pierre (Rapporteur)</authority>
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                <term xml:lang="en">hyperpolarised xenon</term>
                <term xml:lang="en">hydrophobic cavity</term>
                <term xml:lang="en">LTP: lipid transfer protein</term>
                <term xml:lang="en">molecular dynamics</term>
                <term xml:lang="en">molecular and comparative modelling</term>
                <term xml:lang="en">NMR</term>
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                <term xml:lang="fr">modélisation moléculaire et comparative</term>
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                <term xml:lang="fr">LTP : protéines de transfert de lipides</term>
                <term xml:lang="fr">Nicotiana tabacum</term>
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                <term xml:lang="fr">xénon hyperpolarisé&lt;br /&gt;________________________________________________________</term>
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              <p>The LTP1 extracted from leaves of Nicotiana tabacum, named LTP1_1, was produced in Pichia pastoris. We have determined its three-dimensional structure by 2D and 3D NMR and molecular modelling. Various interaction and dynamic studies have highlighted the LTP1_1particular binding properties compared to other LTPs. This work was used as a basis for the structure determination of others tobacco LTP1 using comparative modelling and for molecular dynamic simulations at ambient temperature and high temperature. Moreover, we mapped the LTP1_1 hydrophobic cavity by using hyperpolarized xenon NMR.&lt;br /&gt;This work allowed us to identify the amino acids involved specifically in lipid binding. We have discovered that the remarkable binding properties of LTP1 are related not only on their original structure but also in their particular dynamic.</p>
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              <p>La LTP1 extraite de feuille de Nicotiana tabacum, nommée LTP1_1, a été produite dans Pichia pastoris. Nous avons ainsi déterminé sa structure tridimensionnelle par RMN 2D, 3D et modélisation moléculaire. Différentes études d'interaction et de dynamique ont ensuite mis en évidence des propriétés de fixation particulières comparativement aux autres LTPs. Ces travaux ont servi de base à la détermination de structures d'autres LTP1 de tabac par modélisation comparative et à des simulations de dynamique moléculaire à température ambiante et à haute température. De plus, nous avons cartographié la cavité hydrophobe de la LTP1_1 en utilisant la RMN du xénon hyperpolarisé.&lt;br /&gt;L'ensemble de ces travaux a permis d'identifier les acides aminés impliqués spécifiquement dans la fixation des lipides. Nous avons découvert que les propriétés remarquables de fixation des LTP1 sont liées non seulement à leur structure originale mais également à leur dynamique particulière.</p>
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