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Nanofluidique de solutions polymériques appliquées à la synthèse in situ d'oligosaccharides

Abstract : Biochips are experiencing recently a great success with possible applications for DNA,proteins and oligosaccharides. An oligosaccharide chip presents difficulties compared to aDNA chip because of the many temperature constraints and less work has been performed inthis area. This work is devoted to the study of a oligosaccharide chip fabricated par supportedsynthesis and protected with a polymer film. The manufacturing process is particularlydetailed.We first examine experimentally the formation of a polymer film obtained by evaporation of adrop of polymer solution on a chemically structured surface (zone of different wettability) byfollowing its transient evolution. We show that this type of heterogeneous surface isparticularly suitable for manufacturing biochips. On the other hand, we propose a numericalmodel of the evaporation of a drop of polymer solution on a heated surface using thelubrication method and a “height of resin”. The experimental results and numericalsimulations are compared and show good qualitative agreement on the shape of the polymerfilms obtained after evaporation.In this work, the supported synthesis of oligosaccharide microarrays was carried out usingpolymers and surfaces which have been carefully chosen in preliminary testing. In particular,the masking technique using polymer film turns out to be highly suitable for protectingoligomers at both high and very low temperatures.
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Submitted on : Monday, July 30, 2012 - 11:12:11 AM
Last modification on : Friday, March 25, 2022 - 9:40:25 AM
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  • HAL Id : tel-00721738, version 1




David Rolland. Nanofluidique de solutions polymériques appliquées à la synthèse in situ d'oligosaccharides. Mécanique des fluides [physics.class-ph]. Université de Grenoble, 2012. Français. ⟨NNT : 2012GRENI012⟩. ⟨tel-00721738⟩



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