Les réactifs utilisés pour l'immobilisation des enzymes par réticulation chimique sont le glutaraldéhyde (Sigma®) en solution aqueuse à 25% (rn/v) et l'ASH_à 20% (rn/v) fournie par le Centre National de Transfusion ,
06 g.mol-1 1E:MED (Fluka®), pp.21-22 ,
40% (rn/v) en solution aqueuse FIA transfert maritime posent actuellement de graves problèmes. L'AChE, après avoir subi une inhibition supérieure à 88 %, est réactivée avec une solution diluée de 2-PAM et peut ainsi être réutilisée pour une analyse ultérieure ,
75 mm) sur lesquelles l'AChE est immobilisée sont fournies par Bioblock® Scientific (France), alors que les tubes de verre (SZ!int = 1,1 mm), dans lesquels les billes sont insérées (Figure 4.2) proviennent de Corning® France ,
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A microassay-based procedure for measuring low levels of toxic organophosphorus compounds through acetylcholinesterase inhibition, Analytical Biochemistry, vol.180, issue.2, pp.380-383, 1990. ,
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Spontaneous Reactivation of Acetylcholinesterase Following Organophosphate Inhibition -An Analysis of Anomalous Reactivation Kinetics, Biochem. Biophys. Acta, pp.483312-319, 1977. ,
Sensitive Biosensor for Choline and Acetylcholine Involving Fast Immobilization of a Bienzyme System on a Disposable Membrane, Anal. Chim. Acta, vol.231, pp.27-32, 1990. ,
Palladium-palladium oxide pH electrodes, Analytical Chemistry, vol.52, issue.2, pp.270-273, 1980. ,
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Iridium/Iridium Oxide Electrode for Potentiometric Determination of Proton Activity in Hydroorganic Solutions at Sub-Zero Temperatures, Anal. Chem, vol.56, pp.317-319, 1984. ,
Urea Sensor Based on Iridium Dioxide Electrodes With Immobilized Urease, Anal. Chim. Acta, vol.146, pp.249-253, 1983. ,
Pd/PdO Based Urea and Butyrylcholine Microbiosensors, Anabiotec '90, 3rd Inti, Symposium on Analytical Methods and Problems in Biotechnology, pp.22-24, 1990. ,
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Komplexe von Nonactin Mit Natrium-, Kalium-und Ammonium-Ionen, Hel. Chim. Acta, vol.50, pp.1373-1376, 1967. ,
An Improved Electrode for the Assay ofUrea in Blood, Anal. Chim. Acta, vol.67, pp.195-201, 1973. ,
Purification of Urease From the Seeds of Ca janus Ca jan and the Construction of a Sensor for the Analysis ofUrea in Human Serum, 1991. ,
Tetrathiafulvalene Tetracyanoquinod.imethane Xanthine Oxidase Amperometric Electrode for the Determination of Biological Purines, Anal. Chem, vol.59, pp.954-958, 1987. ,
A Glucose Sensor Utilising Tetracyanoquinodimethane as a Mediator, Hormone & Metabolic Research Supplement Series, pp.37-40, 1988. ,
A Coated Metal Enzyme Electrode for Urea Determinations, Anal. Chim. Acta, vol.131, pp.103-109, 1981. ,
Insecticide Determination With Enzyme Electrodes Using Different Enzyme Immobilisation Techniques, Electroanalysis, 1992. ,
An Enzyme Electrode for the Substrate Urea, J. Amer. Chem. Soc, vol.92, pp.2533-2538, 1970. ,
Micro-Electrode à Enzyme Immobilisée pour la Détection de l'Acétylcholine, Analusis, pp.1325-1354, 1985. ,
Kinetic Analysis of Enzyme Electrode Response, Anal. Chem, vol.56, pp.664-667, 1984. ,
Amperometric Determination of Choline With Use oflmmobilized Choline Oxidase, Biotech. Bioeng, vol.22, pp.1071-1086, 1980. ,
Immobilization of Thin Enzyme Membranes to Construct Glass Enzyme Electrodes, Anal. Chem, vol.63, pp.1914-1918, 1991. ,
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Rapid Measurement of Penicillin Contained in Complex Mediums by Enzyme Loaded Glass Electrodes, Anal. Chim. Acta, pp.249405-411, 1991. ,
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Determinattion ofL-Alanine in a Flow Injection Analysis System With an Immobilized Enzyme Reactor, Anal. Chim. Acta, vol.239, pp.307-310, 1990. ,
An Electroanalytical Method for Estimation ofFish Freshness Using a Flow Injection System With Sorne Immobilized Enzyme Reactors, Electroanalysis, issue.1, pp.173-176, 1989. ,
Short- Term Toxicity Test Using Escherichia Coli: Monitoring C0 2 Production by Flow Injection Analysis, Wat. Res, vol.24, pp.351-354, 1990. ,
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Determination of Glucose in Fermentation Processes by Means of an On-Line Coupled Flow Injection System Using Enzyme Sensors Based on Chemically Modified Electrodes, Anal. Chim. Acta, vol.235, pp.265-271, 1990. ,
Flow injection analysis of pharmaceuticals, Journal of Pharmaceutical and Biomedical Analysis, vol.7, issue.12, pp.1291-1300, 1989. ,
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); Writeln ('Ez5B ,
3); Writeln ,
5); Writeln ('Cls(mM) ,
6); Writeln ('Km (mM) ,
5) Readln (real_Cls); real_Cls := ln(real_Cls)/ln(lO) ,
8); Writeln ('Kmbar ,
Cs-(real)-cs-(calc) ( ,
Cls(real)-Cls(calc ,
extended) :extended; begin ItAbs := ltAbs+l, GotoXY, vol.20, p.34 ,
21); Writeln (rootA:12) ,
9); Writeln (k:3) ,