R. Utilises-pour-l-'1mmob-!-lisation and . Enzymatique, Les réactifs utilisés pour l'immobilisation des enzymes par réticulation chimique sont le glutaraldéhyde (Sigma®) en solution aqueuse à 25% (rn/v) et l'ASH_à 20% (rn/v) fournie par le Centre National de Transfusion

.. M. Hydrate-d-'hydrazine-)-sigma® and .. , 06 g.mol-1 1E:MED (Fluka®), pp.21-22

). Glyoxal-(-sigma®, 40% (rn/v) en solution aqueuse FIA transfert maritime posent actuellement de graves problèmes. L'AChE, après avoir subi une inhibition supérieure à 88 %, est réactivée avec une solution diluée de 2-PAM et peut ainsi être réutilisée pour une analyse ultérieure

L. Billes-de-verre, 75 mm) sur lesquelles l'AChE est immobilisée sont fournies par Bioblock® Scientific (France), alors que les tubes de verre (SZ!int = 1,1 mm), dans lesquels les billes sont insérées (Figure 4.2) proviennent de Corning® France

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P. C. Pandey, C. Tran-minh, S. Kumaran, and F. Lantreibecq, Purification of Urease From the Seeds of Ca janus Ca jan and the Construction of a Sensor for the Analysis ofUrea in Human Serum, 1991.

M. Kenna, K. Toth, A. , and B. , Tetrathiafulvalene Tetracyanoquinod.imethane Xanthine Oxidase Amperometric Electrode for the Determination of Biological Purines, Anal. Chem, vol.59, pp.954-958, 1987.

H. , S. P. Turner, and A. P. , A Glucose Sensor Utilising Tetracyanoquinodimethane as a Mediator, Hormone & Metabolic Research Supplement Series, pp.37-40, 1988.

A. , P. W. Joseph, and J. P. , A Coated Metal Enzyme Electrode for Urea Determinations, Anal. Chim. Acta, vol.131, pp.103-109, 1981.

K. , S. Tran-minh, and C. , Insecticide Determination With Enzyme Electrodes Using Different Enzyme Immobilisation Techniques, Electroanalysis, 1992.

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N. , D. Mungal, R. Ngo, and T. T. , Flow Injection Analysis of Serum Urea Using Urease Covalently Immobilized on 2-Fluoro-1-Methylpyridinium Salt- Activated Fractogel and Fluoroscence Detection, Anal. Biochem, vol.188, pp.325-329, 1990.

K. , N. Tagami, H. Furusawa, and M. , Determinattion ofL-Alanine in a Flow Injection Analysis System With an Immobilized Enzyme Reactor, Anal. Chim. Acta, vol.239, pp.307-310, 1990.

Y. , T. Matsumoto, and Y. , An Electroanalytical Method for Estimation ofFish Freshness Using a Flow Injection System With Sorne Immobilized Enzyme Reactors, Electroanalysis, issue.1, pp.173-176, 1989.

J. , W. F. Pasquin-!, C. Guimaraes, J. R. De-faria, and L. C. , Short- Term Toxicity Test Using Escherichia Coli: Monitoring C0 2 Production by Flow Injection Analysis, Wat. Res, vol.24, pp.351-354, 1990.

H. , S. Schwedt, and G. , Determination of Total Phosphorous in Waters With Amperometric Detection by Coupling of Flow-Injection Analysis With Continuous Microwave Oven Digestion, Anal. Chim. Acta, pp.236345-350, 1990.

A. , S. Alonso, J. Bartroli, J. Del-v-alle, and M. , Flow-Through pH-ISFET as Detecter in the Determination of Ammonia, Anal. Chim. Acta, pp.231-53, 1990.

K. , S. Tran-minh, C. Valero, F. Lafuente, J. Poch et al., Determination of Organophosphorous and Carbamate Insecticides by Flow Injection Analysis Biomass Estimation Using On-Line Glucose Monitoring by Flow Injection Analysis, Anal. Biochem. Appl. Biochem." Biotechnol, vol.24, pp.25591-602, 1990.

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