. Abgrall, A novel fabrication method of flexible and monolithic 3D microfluidic structures using lamination of SU-8 films, Journal of Micromechanics and Microengineering, vol.16, issue.1, pp.113-121, 2006.
DOI : 10.1088/0960-1317/16/1/016

. Alderman, Microfabrication of channels using an embedded mask in negative resist, Journal of Micromechanics and Microengineering, vol.11, issue.6, pp.703-708, 2001.
DOI : 10.1088/0960-1317/11/6/312

. Alshareef, Separation of tumor cells with dielectrophoresis-based microfluidic chip, Biomicrofluidics, vol.7, issue.1, p.11803, 2013.
DOI : 10.1063/1.4774312

E. Taverna and . Dufour-gergam, A new controlled concept of immune-sensing platform for specific detection of Alzheimer's biomarkers, Selected Papers from the World Congress on Biosensors, pp.329-335, 2013.

A. , Z. Arnold, and U. Zimmermann, Electro-rotation?development of a technique for dielectric measurements on individual cells and particles, J. Electrostat, vol.21, issue.2-3, pp.151-191, 1988.

P. Scorsone, L. Bergonzo, S. Chicaud, J. A. Picaud, and . Sahel, A passive pressure sensor for continuously measuring the intraocular pressure in glaucomatous patients, IRBM, vol.33, issue.2, pp.117-122, 2012.
URL : https://hal.archives-ouvertes.fr/lirmm-00685354

. Avignan, Fusion Cell Vaccination of Patients with Metastatic Breast and Renal Cancer Induces Immunological and Clinical Responses, Clinical Cancer Research, vol.10, issue.14, pp.4699-4708, 2004.
DOI : 10.1158/1078-0432.CCR-04-0347

. Ayed, Ichraf Ayed Développement d'une méthode de préconcentration de phosphopeptides sur phase monolithique en puce, Thèse de Doctorat soutenue le 27 Septembre 2012

. Bachi, Fusion of Erythrocytes by Sendai Virus Studied by Immuno-Freeze- Etching, Journal of Virology, vol.11, issue.6, pp.1004-1012, 1973.

. Bai, A double droplet trap system for studying mass transport across a droplet-droplet interface, Lab on a Chip, vol.253, issue.10, pp.1281-1285, 2010.
DOI : 10.1039/b925133b

. Barbuto, Dendritic cell?tumor cell hybrid vaccination for metastatic cancer, Cancer Immunology, Immunotherapy, vol.161, issue.12, pp.1111-1118, 2004.
DOI : 10.1007/s00262-004-0551-7

. Barrau, Osmotically induced membrane tension facilitates the triggering of living cell electropermeabilization, Bioelectrochemistry, vol.63, issue.1-2, pp.327-332, 2004.
DOI : 10.1016/j.bioelechem.2003.11.009

. Becker, Separation of human breast cancer cells from blood by differential dielectric affinity., Proc. Natl. Acad. Sci. USA, pp.860-864, 1995.
DOI : 10.1073/pnas.92.3.860

. Coirault, Les pinces optiques en biologie et en m??decine, m??decine/sciences, vol.19, issue.3, pp.364-367, 2003.
DOI : 10.1051/medsci/2003193364

. Couty, Etude du procédé de réalisation de micro-antennes souples implantables pour l'Imagerie médicale par Résonance Magnétique, 2012.
DOI : 10.3166/i2m.12.1-2.9-35

H. G. Coster, A Quantitative Analysis of the Voltage-Current Relationships of Fixed Charge Membranes and the Associated Property of ???Punch-Through???, Biophysical Journal, vol.5, issue.5, pp.669-686, 1965.
DOI : 10.1016/S0006-3495(65)86745-5

. Cummings, Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results Analytical chemistry, pp.75-4724, 2003.

. Dalmay, Ultra sensitive biosensor based on impedance spectroscopy at microwave frequencies for cell scale analysis, Sensors and Actuators A: Physical, vol.162, issue.2, pp.189-197, 2009.
DOI : 10.1016/j.sna.2010.04.023

URL : https://hal.archives-ouvertes.fr/hal-00583621

. Dalmay, Design and realization of a microfluidic device devoted to the application of ultra-short pulses of electrical field to living cells, Sensors and Actuators B: Chemical, vol.160, issue.1, pp.1573-1580, 2011.
DOI : 10.1016/j.snb.2011.09.009

URL : https://hal.archives-ouvertes.fr/hal-00738711

. Dalmay, A microfluidic device with removable packaging for the real time visualisation of intracellular effects of nanosecond electrical pulses on adherent cells, Lab on a Chip, vol.58, issue.1, pp.4709-4715, 2012.
DOI : 10.1073/pnas.96.8.4262

URL : https://hal.archives-ouvertes.fr/hal-00745203

K. E. Sullivan, J. L. Ugen, R. Messina, and . Heller, Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma, J. Clin. Oncol, vol.26, pp.5896-5903, 2008.

. Davalos, Tissue Ablation with Irreversible Electroporation, Annals of Biomedical Engineering, vol.14, issue.052901, pp.223-231, 2005.
DOI : 10.1007/s10439-005-8981-8

. Delemotte, Modeling Membranes under a Transmembrane Potential, The Journal of Physical Chemistry B, vol.112, issue.18, pp.5547-5550, 2008.
DOI : 10.1021/jp710846y

. Deman, Characterization of C-PDMS electrodes for electrokinetic applications in microfluidic systems, Journal of Micromechanics and Microengineering, vol.21, issue.9, p.95013, 2011.
DOI : 10.1088/0960-1317/21/9/095013

URL : https://hal.archives-ouvertes.fr/hal-00734048

. Demierre, Characterization and optimization of liquid electrodes for lateral dielectrophoresis, Lab Chip, vol.5, issue.12, pp.355-365, 2007.
DOI : 10.1039/B612866A

D. Vries, Effective Migration of Antigen-pulsed Dendritic Cells to Lymph Nodes in Melanoma Patients Is Determined by Their Maturation State, Cancer Res, vol.63, pp.12-17, 2003.

D. Carlo, Dynamic single cell culture array, Lab on a Chip, vol.7, issue.11, pp.1445-1449, 2006.
DOI : 10.1039/b605937f

. Dimova, Giant vesicles in electric fields, Soft Matter, vol.278, issue.7, pp.817-827, 2007.
DOI : 10.1039/b703580b

URL : http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.455.3804

. Djilas, Three-dimensional electrode arrays, 2011.

. Gel, Microorifice-Based High-Yield Cell Fusion on Microfluidic Chip: Electrofusion of Selected Pairs and Fusant Viability, IEEE Transactions on NanoBioscience, vol.8, issue.4, pp.300-305, 2009.
DOI : 10.1109/TNB.2009.2035252

. Gel, Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array, Biomicrofluidics, vol.4, issue.2, p.22808, 2010.
DOI : 10.1063/1.3422544.2

T. Chamalye and . Bourouina, A SAW filter integrated on a silicon passive substrate used for system in package, Sensors and Actuators A, vol.142, pp.185-191, 2008.

. Gimsa, Dielectrophoresis and electrorotation of neurospora slime and murine myeloma cells, Biophysical Journal, vol.60, issue.4, pp.749-760, 1991.
DOI : 10.1016/S0006-3495(91)82109-9

. Gimsa, Analytical Description of the Transmembrane Voltage Induced on Arbitrarily Oriented Ellipsoidal and Cylindrical Cells, Biophysical Journal, vol.81, issue.4, pp.1888-1896, 2001.
DOI : 10.1016/S0006-3495(01)75840-7

. Glass, Organosilane deposition for microfluidic applications, Biomicrofluidics, vol.5, issue.3, p.36501, 2011.
DOI : 10.1063/1.3625605

. Goddard, In vitro dendritic cell-induced T cell responses to B cell chronic lymphocytic leukaemia enhanced by IL-15 and dendritic cell-B-CLL electrofusion hybrids, Clinical and Experimental Immunology, vol.32, issue.1, pp.82-89, 2003.
DOI : 10.1038/73193

. Golzio, Control by Osmotic Pressure of Voltage-Induced Permeabilization and Gene Transfer in Mammalian Cells, Biophysical Journal, vol.74, issue.6, pp.3015-3022, 1998.
DOI : 10.1016/S0006-3495(98)78009-9

. Gong, Induction of antitumor activity by immunization with fusions of dendritic and carcinoma cells, Nature Medicine, vol.36, issue.5, pp.558-561, 1997.
DOI : 10.1038/nm1295-1297

. Gray, Dielectrophoretic registration of living cells to a microelectrode array, Biosensors and Bioelectronics, vol.19, issue.7, pp.771-780, 2004.
DOI : 10.1016/j.bios.2003.08.013

. Gregoire, Alliages dentaires, Société Francophone des Biomatériaux Dentaires, 2009.

. Guerin, Simple and low cost fabrication of embedded microchannels by using a new thick-film photoplastic, Solid State Sensors and ActuatorsTRANSDUCERS '97, international conference, pp.1419-1422, 1997.

. Halfmann, Transfer of mitochondrial function into a cytoplasmic respiratory-deficient mutant of Saccharomyces yeast by electro-fusion, Current Genetics, vol.7, issue.1, pp.25-28, 1982.
DOI : 10.1007/BF00397637

. Haluska, Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution, Proceedings of the National Academy of Sciences, vol.103, issue.43, pp.15841-15846, 2006.
DOI : 10.1073/pnas.0602766103

. Hamdi, A microfluidic biochip dedicated to highly parallelized electrofusion, Proceedings of MMB 2011, pp.4-06, 2011.
URL : https://hal.archives-ouvertes.fr/hal-00739182

. Hamdi, Self-control of cell electroporation by dielectrophoresis transistion, International NanoBioTech, pp.14-16, 2011.

. Jokinen, Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers, Biomicrofluidics, vol.6, issue.1, p.16501, 2012.
DOI : 10.1063/1.3673251

:. T. Jones-1995 and . Jones, Electromechanics of particles, 1995.
DOI : 10.1017/CBO9780511574498

T. B. Jones, Basic theory of dielectrophoresis and electrorotation, IEEE Engineering in Medicine and Biology Magazine, vol.22, issue.6, pp.33-42, 2003.
DOI : 10.1109/MEMB.2003.1304999

. Joshi, Silanization and antibody immobilization on SU-8, Applied Surface Science, vol.253, issue.6, pp.3127-3132, 2007.
DOI : 10.1016/j.apsusc.2006.07.017

. Jothimuthu, Photodefinable PDMS thin films for microfabrication applications, Journal of Micromechanics and Microengineering, vol.19, issue.4, p.45024, 2009.
DOI : 10.1088/0960-1317/19/4/045024

. Ju, An electrofusion chip with a cell delivery system driven by surface tension, Journal of Micromechanics and Microengineering, vol.19, issue.1, p.15004, 2009.
DOI : 10.1088/0960-1317/19/1/015004

. Ju, A microfluidic Device for Electrofusion of Two Plant Cells, World Congress on Medical Physics and Biomedical Engineering, pp.302-305, 2006.
DOI : 10.1007/978-3-540-36841-0_84

. Kao, Superior efficacy of dendritic cell-tumor fusion vaccine compared with tumor lysate-pulsed dendritic cell vaccine in colon cancer, Immunology Letters, vol.101, issue.2, pp.154-159, 2005.
DOI : 10.1016/j.imlet.2005.05.006

. Kato, Cell activation by CpG ODN leads to improved electrofusion in hybridoma production, Journal of Immunological Methods, vol.373, issue.1-2, pp.102-110, 2011.
DOI : 10.1016/j.jim.2011.08.008

. Kemna, On chip electrofusion of single human B cells and mouse myeloma cells for efficient hybridoma generation, ELECTROPHORESIS, vol.336, issue.22, pp.3138-3146, 2011.
DOI : 10.1002/elps.201100227

. Khoshmanesh, Dielectrophoretic platforms for bio-microfluidic systems, Biosensors and Bioelectronics, vol.26, issue.5, pp.1800-1814, 2011.
DOI : 10.1016/j.bios.2010.09.022

. Kikuchi, Results of a phase I clinical trial of vaccination of glioma patients with fusions of dendritic and glioma cells, Cancer Immunology, Immunotherapy, vol.50, issue.7, pp.337-344, 2001.
DOI : 10.1007/s002620100205

. Kimura, Dielectrophoresis-assisted massively parallel cell pairing and fusion based on field constriction created by a micro-orifice array sheet, ELECTROPHORESIS, vol.4, issue.18, pp.2496-2501, 2011.
DOI : 10.1002/elps.201100129

. Koh, Quantitative Studies on PDMS-PDMS Interface Bonding with Piranha Solution and its Swelling Effect, Micromachines, vol.3, issue.4, pp.427-441, 2012.
DOI : 10.3390/mi3020427

M. G. Kohler, C. Kohler, and . Milstein, Continuous cultures of fused cells secreting antibody of predefined specificity, Nature, vol.3, issue.5517, pp.495-497, 1975.
DOI : 10.1038/256495a0

L. , L. Ling, and K. Lian, In situ fabrication of SU-8 movable parts by using PAG-diluted SU8 as the sacrificial layer, Microsyst Technol, vol.13, pp.253-257, 2007.

. Mahaworasilpa, Forces on biological cells due to applied alternating (AC) electric fields. II. Electro-rotation, Biochimica et Biophysica Acta (BBA) - Biomembranes, vol.1281, issue.1, pp.5-14, 1996.
DOI : 10.1016/0005-2736(95)00271-5

. Markin, On the theory of membrane fusion. The stalk mechanism, Gen Physiol Biophys, vol.3, issue.5, pp.361-77, 1984.

I. G. Sauerbruch and . Schmidt-wolf, Allogeneic Dendritic Cells Fused with Tumor Cells: Preclinical Results and Outcome of a Clinical Phase I/II Trial in Patients with Metastatic Renal Cell Carcinoma, Hum Gene Ther, vol.20, issue.14, pp.483-94, 2003.

. Masuda, Novel method of cell fusion in field constriction area in fluid integration circuit, IEEE Transactions on Industry Applications, vol.25, issue.4, pp.732-737, 1989.
DOI : 10.1109/28.31255

. Metz, Polyimide and SU-8 microfluidic devices manufactured by heat-depolymerizable sacrificial material technique, Lab on a Chip, vol.4, issue.2, pp.114-120, 2004.
DOI : 10.1039/b310866j

. Mir, H. Mir, C. Banoun, and . Paoletti, Introduction of definite amounts of nonpermeant molecules into living cells after electropermeabilization: Direct access to the cytosol, Experimental Cell Research, vol.175, issue.1, pp.15-25, 1988.
DOI : 10.1016/0014-4827(88)90251-0

C. Luboinski and . Paoletti, Electrochemotherapy, a new antitumor treatment : first clinical trial, C.R. Acad. Sci. III, vol.313, pp.613-618, 1991.

. Mir, Electrochemotherapy potentiation of antitumour effect of bleomycin by local electric pulses, European Journal of Cancer and Clinical Oncology, vol.27, issue.1, pp.68-72, 1991.
DOI : 10.1016/0277-5379(91)90064-K

. Mir, Electric Pulse???Mediated Gene Delivery to Various Animal Tissues, Adv. Genet, vol.54, pp.83-114, 2005.
DOI : 10.1016/S0065-2660(05)54005-7

URL : https://hal.archives-ouvertes.fr/hal-00319942

O. Sullivan and M. Marty, Standard operating procedures of the electrochemotherapy : Instructions for the use of bleomycin or cisplatin administered either systemically or locally and electric pulses delivered by the cliniporator by means of invasive or non-invasive electrodes, Eur. J. Cancer, vol.4, pp.14-25, 2006.

. Moisescu, Changes of cell electrical parameters induced by electroporation. A dielectrophoresis study, Biochimica et Biophysica Acta (BBA) - Biomembranes, vol.1828, issue.2, pp.365-372, 2012.
DOI : 10.1016/j.bbamem.2012.08.030

. Morgan, Separation of Submicron Bioparticles by Dielectrophoresis, Biophysical Journal, vol.77, issue.1, pp.516-525, 1999.
DOI : 10.1016/S0006-3495(99)76908-0

M. Et-green, H. Morgan, and N. G. Green, AC Electrokinetics: Colloids and Nanoparticles, 2003.

. Mottet, A technique to design complex 3D lab on a chip involving multilayered fluidics, embedded thick electrodes and hard packaging???application to dielectrophoresis and electroporation of cells, Journal of Micromechanics and Microengineering, vol.20, issue.4, p.47001, 2010.
DOI : 10.1088/0960-1317/20/4/047001

URL : https://hal.archives-ouvertes.fr/hal-00592400

. Mottet, High-resolution analyses of cell fusion dynamics in a biochip, ELECTROPHORESIS, vol.40, issue.16, pp.2508-2515, 2012.
DOI : 10.1002/elps.201200112

URL : https://hal.archives-ouvertes.fr/hal-00738706

. Muller, Reversible Electropermeabilization of Mammalian Cells by High-Intensity, Ultra-Short Pulses of Submicrosecond Duration, Journal of Membrane Biology, vol.184, issue.2, pp.161-170, 2001.
DOI : 10.1007/s00232-001-0084-3

. Murillo, Novel SU-8 based vacuum wafer-level packaging for MEMS devices, The 35th International Conference on Micro-and Nano-Engineering (MNE)), pp.1173-1176, 2010.
DOI : 10.1016/j.mee.2009.12.048

. Neumann, Permeability changes induced by electric impulses in vesicular membranes, The Journal of Membrane Biology, vol.22, issue.1, pp.279-290, 1972.
DOI : 10.1007/BF01867861

. Neumann, Biophysical considerations of membrane electroporation -Guide to electroporation and electrofusion, AcademicPress, pp.77-90, 1992.

. Oh, Comprehensive analysis of particle motion under non-uniform AC electric fields in a microchannel, Lab Chip, vol.89, issue.1, pp.62-78, 2009.
DOI : 10.1039/B801594E

. Oh, Design of pressure-driven microfluidic networks using electric circuit analogy, Lab Chip, vol.7, issue.3, pp.515-545, 2012.
DOI : 10.1039/C2LC20799K

. Orlowski, Transient electropermeabilization of cells in culture, Biochemical Pharmacology, vol.37, issue.24, pp.4727-4733, 1988.
DOI : 10.1016/0006-2952(88)90344-9

. Ozil, Effects of electric field on fusion rate and survival of 2-cell rabbit embryos, J. Embryol. Exp. Morph, vol.96, pp.211-228, 1986.

. Parkhurst, Hybrids of Dendritic Cells and Tumor Cells Generated by Electrofusion Simultaneously Present Immunodominant Epitopes from Multiple Human Tumor-Associated Antigens in the Context of MHC Class I and Class II Molecules, The Journal of Immunology, vol.170, issue.10, pp.5317-5325, 2003.
DOI : 10.4049/jimmunol.170.10.5317

. Patel, Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis, Biomicrofluidics, vol.6, issue.3, p.34102, 2012.
DOI : 10.1063/1.4732800.2

S. Pauly, ??ber die Impedanz einer Suspension von kugelf??rmigen Teilchen mit einer Schale, Zeitschrift f??r Naturforschung B, vol.14, issue.2, pp.125-131, 1959.
DOI : 10.1515/znb-1959-0213

. Pethig, Enhancing traveling-wave dielectrophoresis with signal superposition, IEEE Engineering in Medicine and Biology Magazine, vol.22, issue.6, pp.43-50, 2003.
DOI : 10.1109/MEMB.2003.1266046

. Poignard, Ion fluxes, transmembrane potential, and osmotic stabilization: a new dynamic electrophysiological model for eukaryotic cells, European Biophysics Journal, vol.452, issue.1, pp.235-246, 2010.
DOI : 10.1007/s00249-010-0641-8

. Prabhakar, A novel C-shaped, gold nanoparticle coated, embedded polymer waveguide for localized surface plasmon resonance based detection, Lab on a Chip, vol.11, issue.4, pp.3422-3425, 2010.
DOI : 10.1039/c005253a

. Pucihar, The influence of medium conductivity on electropermeabilization and survival of cells in vitro, Bioelectrochemistry, vol.54, issue.2, pp.107-115, 2001.
DOI : 10.1016/S1567-5394(01)00117-7

. Pucihar, The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy, Bioelectrochemistry, vol.57, issue.2, pp.167-172, 2002.
DOI : 10.1016/S1567-5394(02)00116-0

URL : https://hal.archives-ouvertes.fr/hal-00320042

. Pucihar, Kinetics of Transmembrane Transport of Small Molecules into Electropermeabilized Cells, Biophysical Journal, vol.95, issue.6, pp.2837-2848, 2008.
DOI : 10.1529/biophysj.108.135541

M. A. Unger, H. Chou, T. Thorsen, A. Scherer, and S. R. Quake, Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography, Science, vol.288, issue.5463, pp.113-116, 2000.
DOI : 10.1126/science.288.5463.113

T. Thorsen, R. W. Roberts, F. H. Arnold, and S. R. Quake, Dynamic Pattern Formation in a Vesicle-Generating Microfluidic Device, Physical Review Letters, vol.86, issue.18, pp.4163-4166, 2001.
DOI : 10.1103/PhysRevLett.86.4163

. Bosseboeuf, Effects of direct and pulse current on copper electrodeposition through photoresist molds, J. Micromech. Microeng, vol.10, pp.116-119, 2000.

. Ramadan, Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device, Sensors and Actuators B: Chemical, vol.113, issue.2, pp.944-955, 2006.
DOI : 10.1016/j.snb.2005.04.018

. Ramos, Ac electrokinetics: a review of forces in microelectrode structures, Journal of Physics D: Applied Physics, vol.31, issue.18, pp.2338-2353, 1998.
DOI : 10.1088/0022-3727/31/18/021

. Ramos, AC Electric-Field-Induced Fluid Flow in Microelectrodes, Journal of Colloid and Interface Science, vol.217, issue.2, pp.420-422, 1999.
DOI : 10.1006/jcis.1999.6346

:. C. Ramos-2000 and . Ramos, Etude des mécanismes de fusion cellulaire : approche biophysique par electropulsation, 2000.

. Regtmeier, Dielectrophoretic Manipulation of DNA:?? Separation and Polarizability, Analytical Chemistry, vol.79, issue.10, pp.3925-3932, 2007.
DOI : 10.1021/ac062431r

D. Riske, Electric Pulses Induce Cylindrical Deformations on Giant Vesicles in Salt Solutions, Biophysical Journal, vol.91, issue.5, pp.1778-1786, 2006.
DOI : 10.1529/biophysj.106.081620

. Riske, ELECTROFUSION OF MODEL LIPID MEMBRANES VIEWED WITH HIGH TEMPORAL RESOLUTION, Biophysical Reviews and Letters, vol.01, issue.04, pp.387-400, 2006.
DOI : 10.1142/S179304800600032X

R. Et-teissié, M. Rols, and J. Teissie, Modulation of electrically induced permeabilization and fusion of Chinese hamster ovary cells by osmotic pressure, Biochemistry, vol.29, pp.4561-4567, 1990.

. Rols, In vivo electrically mediated protein and gene transfer in murine melanoma, Nature Biotechnology, vol.1334, issue.2, pp.168-171, 1998.
DOI : 10.1006/abio.1976.9999

. Sale, Effects of high electric fields on micro-organisms, Biochimica et Biophysica Acta (BBA) - Biomembranes, vol.163, issue.1, pp.37-43, 1968.
DOI : 10.1016/0005-2736(68)90030-8

. Salomon, A dielectrophoretic continuous flow sorter using integrated microelectrodes coupled to a channel constriction, ELECTROPHORESIS, vol.61, issue.12, pp.1508-1514, 2011.
DOI : 10.1002/elps.201000675

. Salomon, Arrays of nanoelectromechanical biosensors functionalized by microcontact printing, Nanotechnology, vol.23, issue.49, p.495501, 2012.
DOI : 10.1088/0957-4484/23/49/495501

. Schaper, Development of a Technology for Arranged Electrofusion of Mammalian Cells: Applicability in Breast Cancer Immunotherapy, 2007.

. Schaumacher, Hydrophobic coating of microfluidic chips structured by SU-8 polymer for segmented flow operation, Journal of Micromechanics and Microengineering, vol.18, issue.5, p.550196, 2008.
DOI : 10.1088/0960-1317/18/5/055019

. Schoenbach, Intracellular effect of ultrashort electrical pulses, Bioelectromagnetics, vol.1, issue.6, pp.440-448, 2001.
DOI : 10.1002/bem.71

. Schoenbach, Bioelectric Effects of Intense Nanosecond Pulses, IEEE Transactions on Dielectrics and Electrical Insulation, vol.14, issue.5, p.1088, 2007.
DOI : 10.1109/TDEI.2007.4339468

. Schwartzenberger, Impedance sensor technology for cell-based assays in the framework of a high-content screening system, Physiological Measurement, vol.32, issue.7, pp.977-993, 2011.
DOI : 10.1088/0967-3334/32/7/S18

. Seichepine, A combination of capillary and dielectrophoresis-driven assembly methods for wafer scale integration of carbon-nanotube-based nanocarpets, Nanotechnology, vol.23, issue.9, p.95303, 2012.
DOI : 10.1088/0957-4484/23/9/095303

URL : https://hal.archives-ouvertes.fr/hal-00832109

:. L. Sharpe-1963, H. Sharpe, and . Schonhorn, Theory gives direction to adhesion work, Chemical and Engineering News, vol.41, issue.15, pp.67-68, 1963.

. Shim, Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems, Biomicrofluidics, vol.7, issue.1, p.11807, 2013.
DOI : 10.1063/1.4774307

. Siders, Induction of specific antitumor immunity in the mouse with the electrofusion product of tumor cells and dendritic cells, Molecular Therapy, vol.7, issue.4, pp.498-505, 2003.
DOI : 10.1016/S1525-0016(03)00044-3

. Silve, Control of current intensity: Experimental proofs of the relevance of current density in biological cells permeabilisation caused by nanosecond electric pulses, 2011 IEEE Pulsed Power Conference, 2011.
DOI : 10.1109/PPC.2011.6191681

. Sigurdson, Electrothermal stirring for heterogeneous immunoassays, Lab on a Chip, vol.31, issue.4, pp.1366-1373, 2005.
DOI : 10.1039/b508224b

. Skelley, Microfluidic control of cell pairing and fusion, Nature Methods, vol.6, issue.2, pp.147-152, 2009.
DOI : 10.1016/j.biomaterials.2007.03.023

. Sowers, A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses, The Journal of Cell Biology, vol.102, issue.4, pp.1358-1362, 1986.
DOI : 10.1083/jcb.102.4.1358

. Squires, Induced-charge electro-osmosis, Journal of Fluid Mechanics, vol.509, pp.217-252, 2004.
DOI : 10.1017/S0022112004009309

. Sukhorukov, The Effect of Electrical Deformation Forces on the Electropermeabilization of Erythrocyte Membranes in Low- and High-Conductivity Media, Journal of Membrane Biology, vol.163, issue.3, pp.235-245, 1998.
DOI : 10.1007/s002329900387

. Sukhorukov, A biophysical approach to the optimisation of dendritic-tumour cell electrofusion, Biochemical and Biophysical Research Communications, vol.346, issue.3, pp.829-839, 2006.
DOI : 10.1016/j.bbrc.2006.05.193

. Sunkara, Simple room temperature bonding of thermoplastics and poly(dimethylsiloxane), Lab Chip, vol.288, issue.5, pp.962-965, 2011.
DOI : 10.1039/C0LC00272K

. Talaei, Hybrid microfluidic cartridge formed by irreversible bonding of SU-8 and PDMS for multi-layer flow applications, Proceedings of the Eurosensors XXIII conference, pp.381-384, 2009.
DOI : 10.1016/j.proche.2009.07.095

. Tanaka, Therapeutic immune response induced by electrofusion of dendritic and tumor cells, Cellular Immunology, vol.220, issue.1, pp.1-12, 2002.
DOI : 10.1016/S0008-8749(03)00009-1

. Tarek, Membrane Electroporation: A Molecular Dynamics Simulation, Biophysical Journal, vol.88, issue.6, pp.4015-4053, 2005.
DOI : 10.1529/biophysj.104.050617

. Velugotla, Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives, Biomicrofluidics, vol.6, issue.4, p.44113, 2012.
DOI : 10.1063/1.4771316

. Vernier, Nanoelectropulse-driven membrane perturbation and small molecule permeabilization, BMC Cell Biology, vol.7, issue.1, p.37, 2006.
DOI : 10.1186/1471-2121-7-37

. Vernier, Nanosecond Field Alignment of Head Group and Water Dipoles in Electroporating Phospholipid Bilayers, The Journal of Physical Chemistry B, vol.111, issue.45, pp.12993-12996, 2007.
DOI : 10.1021/jp077148q

. Vernier, Life cycle of an electropore: field-dependent and field-independent steps in pore creation and annihilation, The Journal of Membrane Biology, vol.236, issue.1, pp.27-36, 2010.

. Vézy, Simple method for reversible bonding of a polydimethylsiloxane microchannel to a variety of substrates, Micro & Nano Letters, vol.6, issue.10, pp.871-873, 2011.
DOI : 10.1049/mnl.2011.0492

. Villemejane, Julien Villemejane Etude des effets d'impulsions électriques ultra-courtes sur des cellules vivantes, 2010.

R. W. Morfill and . Stark, Surface hydrophilization of SU-8 by plasma and wet chemical processes, Surf. Interface Anal, vol.42, pp.1735-1744, 2010.

L. Wang, Microfluidic cell fusion under continuous direct current voltage, Applied Physics Letters, vol.89, issue.23, p.234102, 2006.
DOI : 10.1063/1.2402122

. Wang, A systematic investigation into the electrical properties of single HeLa cells via impedance measurements and COMSOL simulations, Biosensors and Bioelectronics, vol.24, issue.9, pp.2830-2835, 2009.
DOI : 10.1016/j.bios.2009.02.012

. Washizu, Cell membrane voltage during electrical cell fusion calculated by re-expansion method, Journal of Electrostatics, vol.65, issue.9, pp.555-561, 2007.
DOI : 10.1016/j.elstat.2006.12.001

K. Williamson, L. Meyer, J. Evans, J. J. Martin, S. Stephenson et al., Combined treatment of dendritoma vaccine and low-dose interleukin-2 in stage IV renal cell carcinoma patients induced clinical response: A pilot study, Oncology Reports, vol.18, issue.3, 2007.

. Whitesides, Magnetic separations in biotechnology, Trends in Biotechnology, vol.1, issue.5, pp.144-148, 1983.
DOI : 10.1016/0167-7799(83)90005-7

. Whitesides, Rapid Prototyping of Complex Structures with Feature Sizes Larger Than 20 µm, Adv. Muter, vol.8, issue.11, pp.917-919, 1996.

. Whitesides, Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane), Anal. Chem, vol.70, issue.23, pp.4974-4984, 1998.

G. M. Whitesides and . Whitesides, Fabrication of Topologically Complex Three-Dimensional Microfluidic Systems in PDMS by Rapid Prototyping, Anal. Chem, vol.72, issue.14, pp.3158-3164, 2000.

. Whitesides, Poly(dimethylsiloxane) as a Material for Fabricating Microfluidic Devices, Accounts of Chemical Research, vol.35, issue.7, pp.491-499, 2002.

. Wilmut, Viable offspring derived from fetal and adult mammalian cells, Nature, vol.385, issue.6619, pp.810-813, 1997.
DOI : 10.1038/385810a0

. Woytasik, Fabrication of planar and three-dimensional microcoils on flexible substrates, Microsystem Technologies, vol.12, issue.Part 1, pp.973-978, 2006.
DOI : 10.1007/s00542-006-0135-x

. Wu, Microfluidic self-assembly of tumor spheroids for anticancer drug discovery, Biomedical Microdevices, vol.37, issue.2, pp.197-202, 2008.
DOI : 10.1007/s10544-007-9125-8

. Wu, Determination of electric field threshold for electrofusion of erythrocyte ghosts. Comparison of pulse-first and contact-first protocols, Biophysical Journal, vol.61, issue.3, pp.810-815, 1992.
DOI : 10.1016/S0006-3495(92)81885-4

. Yalcin, On-demand particle enrichment in a microfluidic channel by a locally controlled floating electrode, Sensors and Actuators B: Chemical, vol.153, issue.1, pp.277-283, 2011.
DOI : 10.1016/j.snb.2010.10.025

. Yasuda, Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma, Cancer Immunology, Immunotherapy, vol.101, issue.7, pp.1025-1036, 2007.
DOI : 10.1007/s00262-006-0252-5

Z. Yeh, Nonfouling Hydrophilic Poly(ethylene glycol) Engraftment Strategy for PDMS/SU-8 Heterogeneous Microfluidic Devices, Langmuir, vol.28, issue.46, pp.16227-16236, 2012.
DOI : 10.1021/la303196m

. Zahn, Electromagnetic Field Theory : A Problem Solving Approach, p.746, 1979.

Y. Zhang, A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis, Biomicrofluidics, vol.3, issue.4, p.44105, 2009.
DOI : 10.1063/1.3259628

. Zhang, The fabrication of polymer microfluidic devices using a solid to solid iterfacial polyaddition, 2009.

. Zhang, Sealing SU8 microfluidic channels using PDMS, Biomicrofluidics, vol.5, p.4650, 2011.
DOI : 10.1063/1.3659016

URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364813

. Zhu, Global Analysis of Protein Activities Using Proteome Chips, Science, vol.293, issue.5537, pp.2101-2105, 2001.
DOI : 10.1126/science.1062191

. Zimmermann, Dielectric Breakdown of Cell Membranes, Biophysical Journal, vol.14, issue.11, pp.881-899, 1974.
DOI : 10.1016/S0006-3495(74)85956-4

. Zimmermann, Electric field-induced cell-to-cell fusion, The Journal of Membrane Biology, vol.6, issue.No. 1, pp.165-182, 1982.
DOI : 10.1007/BF01868659

S. Hamdi, O. Français, F. Subra, W. Wang, M. Woytasik et al., Study of the transmembrane potential distribution of cell pairs in a microfluidic device using polymer obstacles to initiate electrofusion " , The Eur. Phys, J. Appl. Phys, vol.62, issue.1, p.11202, 2013.

F. S. Hamdi, O. Français, F. Subra, E. Dufour-gergam, and B. L. Pioufle, Microarray of nonconnected gold pads used as high density electric traps for parallelized pairing and fusion of cells, Biomicrofluidics, vol.7, issue.04410114, 2013.
URL : https://hal.archives-ouvertes.fr/hal-00861239

. Communications-avec-acte-)-n, . Pham, F. Van, G. Hamdi, J. Mottet et al., A high density microfluidic device for cell pairing and electrofusion, Procedia Engineering, p.49, 2010.

B. L. Gergam and . Pioufle, A microfluidic biochip dedicated to highly parallelized electrofusion, Microtechnologies for Medicine and Biology, p.6
URL : https://hal.archives-ouvertes.fr/hal-00739182

F. S. Hamdi, O. Français, M. Woytasik, E. Dufour-gergam, and B. L. Pioufle, Self control of cell electroporation by dielectrophoresis transistion, NanoBioTech

F. S. Hamdi, O. Français, E. Dufour-gergam, and B. L. Pioufle, Biodevices for Cell Electrofusion, pp.978-961, 2012.