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Microencapsulation de protéines dans des systèmes polymériques par des procédés sans solvants toxiques, en particulier la technologie des fluides supercritiques

Abstract : To date, protein encapsulation for sustained-release purpose remains a challenge in the field of microencapsulation. Owing to many benefits that it can offer to patient comfort and therapeutic efficiency, protein encapsulation in drug delivery systems has been drawing the attention of reseachers since decades. Protein encapsulation in polymeric systems such as PLGA (poly-lactic-glycolic-acid) particles has been proven to be an useful approach to attain this objective. Many protein encapsulation methods have been developed; however, the main drawback in these methods lies in the use of volatile toxic solvents, which are considered toxic for the body and the environment . The goal of this work was then to elaborate formulation strategies to find ways of avoiding the use of volatile toxic solvents. Water-miscible non-volatile low-toxic solvents such as glycofurol, isosorbide dimethyl ether were chosen to be used in this work. Pressurized CO2, with its highly flexible properties in fuction of pressure and temperature, was made use of for the development of these new processes. Two formulation methods have been used: the phenomenon of phase separation and the emulsification/extraction method. Different processes for protein encapsulation were developed during this work. Spherical polymeric PLGA particles were successfully generated with satisfactory encapsulation yield of protein (65-80%). Depending on the chosen process, particle size can range from 0.3 to 30 μm. Details in the formulation of PLGA particles for protein encapsulation are presented and the mechanism of particle formation is discussed. Experimental design was used to ascertain the influence of operating factors on the encapsulation yield and to better predict the output in the chosen experimental space.
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https://theses.hal.science/tel-00952800
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Submitted on : Thursday, February 27, 2014 - 3:11:26 PM
Last modification on : Wednesday, October 20, 2021 - 3:19:19 AM

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  • HAL Id : tel-00952800, version 1

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Mykien Tran. Microencapsulation de protéines dans des systèmes polymériques par des procédés sans solvants toxiques, en particulier la technologie des fluides supercritiques. Pharmacologie. Université d'Angers, 2013. Français. ⟨NNT : ⟩. ⟨tel-00952800⟩

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