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Protéines à motif tripartite (TRIM) chez le porc (Sus scrofa) et réplication du rétrovirus endogène porcin

Abstract : From studies of pathogens and their host interaction has emerged the concept of viral restriction considered to be part of an innate immune system. These factors contribute to the control of endogenous retroviruses (ERV) whose emergence may be associated with several diseases such as leukemia or immunodeficiency. Three subgroups of the porcine ERV-γ-1 group (PERV) are replicative. Nevertheless, these PERVs are not associated with any pathology in the pig. Several studies have been performed on viral restriction mechanism capabilities of the pig but these covered a very limited number of restriction factors. Regarding the porcine tripartite motif-containing (poTRIM) proteins, knowledge is weak although several members of this family have proved to be implicated in the viral restriction of other species. The purpose of this study is to investigate the relationship between these orthologous poTRIMs proteins and replicating PERVs. In order to explore this potential interaction, a TRIM protein expressing model in human cells, known to be sensitive to the PERV infection, has been developed. It has enabled us to assess and characterize potential TRIMs effects on the PERV infection cycle. We equally identified poTRIM8 as a restriction factor. Conversely, poTRIM44 seems to act as an enhancer of the PERV infection, while, TRIM11 displayed ambiguous effects including an enhancer effect of the early infectious stages and an inhibitor activity of the late infectious stages. In this study, we also confirmed the PERV insensitivity to the porcine TRIM5α protein. Finally, this work aims at contributing to the understanding of the relationship between PERV replication and their control leading by the host cells.
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Submitted on : Saturday, May 17, 2014 - 1:42:09 PM
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Antonin Demange. Protéines à motif tripartite (TRIM) chez le porc (Sus scrofa) et réplication du rétrovirus endogène porcin. Sciences agricoles. Université Rennes 1, 2013. Français. ⟨NNT : 2013REN1S159⟩. ⟨tel-00992386⟩

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