K. Y. Aguilera and R. A. Brekken, Hypoxia Studies with Pimonidazole in vivo, Bioprotocol, vol.4, 2014.

C. Allemani, Global surveillance of cancer survival 1995-2009: analysis of individual data for 25,676,887 patients from 279 population-based registries in 67 countries (CONCORD-2), Lancet, issue.9972, pp.977-1010, 2015.
URL : https://hal.archives-ouvertes.fr/hal-01912088

P. M. Aponte and A. Caicedo, Stemness in Cancer: Stem Cells, Cancer Stem Cells, and Their Microenvironment, Stem Cells International, pp.1-17, 2017.

J. Bartkova, Oncogene-induced senescence is part of the tumorigenesis barrier imposed by DNA damage checkpoints, Nature, vol.444, issue.7119, pp.633-637, 2006.

I. Ben-porath, An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors, Nature Genetics, vol.40, issue.5, pp.499-507, 2008.

Y. Chen, Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer, Biochemical and biophysical research communications, vol.385, issue.3, pp.307-320, 2009.

F. Chibon, Validated prediction of clinical outcome in sarcomas and multiple types of cancer on the basis of a gene expression signature related to genome complexity, Nature Medicine, vol.16, issue.7, pp.781-787, 2010.

F. X. Dieudonné, High Wnt signaling represses the proapoptotic proteoglycan syndecan-2 in osteosarcoma cells, Cancer Research, vol.70, issue.13, pp.5399-5408, 2010.

F. X. Dieudonné, Targeted inhibition of T-cell factor activity promotes syndecan-2 expression and sensitization to doxorubicin in osteosarcoma cells and bone tumors in mice, Journal of Bone and Mineral Research, vol.27, pp.2118-2129, 2012.

L. A. Doyle, Sarcoma classification: An update based on the 2013 World Health Organization Classification of Tumors of Soft Tissue and Bone, Cancer, vol.120, issue.12, pp.1763-1774, 2014.

C. A. Eide and B. J. Druker, Understanding cancer from the stem cells up, Nature Medicine, vol.23, issue.6, pp.656-657, 2017.

H. Fujii, Sphere-forming stem-like cell populations with drug resistance in human sarcoma cell lines, International journal of oncology, vol.34, issue.5, pp.1381-1387, 2009.

X. Gao, A proposed quantitative index for assessing the potential contribution of reprogramming to cancer stem cell kinetics, Stem cells international, p.249309, 2014.

L. García-prat, Autophagy maintains stemness by preventing senescence, Nature, vol.529, issue.7584, pp.37-42, 2016.

C. P. Gibbs, Stem-like cells in bone sarcomas: implications for tumorigenesis, Neoplasia, vol.7, issue.11, pp.967-76, 2005.

G. V. Glinsky, Genomics Law Governs Clinical Behavior of Human Cancer: Implications for Decision Making in Disease Management, Journal of Clinical Oncology, vol.26, issue.17, pp.2846-2853, 2008.

M. J. Grimshaw, Mammosphere culture of metastatic breast cancer cells enriches for tumorigenic breast cancer cells, Breast Cancer Research, vol.10, issue.3, 2008.

A. He, CD133 expression predicts lung metastasis and poor prognosis in osteosarcoma patients: A clinical and experimental study, vol.4, pp.435-441, 2012.

M. Höckel and P. Vaupel, Tumor hypoxia: definitions and current clinical, biologic, and molecular aspects, Journal of the National Cancer Institute, vol.93, issue.4, pp.266-76, 2001.

C. Kang, The DNA damage response induces inflammation and senescence by inhibiting autophagy of GATA4, Science, issue.6255, p.5612, 2015.

S. Lee, Increased expression of calpain 6 in uterine sarcomas and carcinosarcomas: an immunohistochemical analysis, International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, vol.17, issue.1, pp.248-53, 2017.

T. Lesluyes, RNA sequencing validation of the Complexity INdex in SARComas prognostic signature, European Journal of Cancer, vol.57, pp.104-111, 2016.

Y. Liu, miR-449a promotes liver cancer cell apoptosis by downregulation of Calpain 6 and POU2F1, Oncotarget, vol.7, issue.12, pp.13491-501, 2016.

J. A. Magee, E. Piskounova, and S. J. Morrison, Cancer stem cells: impact, heterogeneity, and uncertainty, Cancer cell, vol.21, issue.3, pp.283-96, 2012.

A. Marion, Calpain-6 is an endothelin-1 signaling dependent protective factor in chemoresistant osteosarcoma, International journal of cancer, vol.130, issue.11, pp.2514-2539, 2012.

J. Mathieu, HIF induces human embryonic stem cell markers in cancer cells, Cancer research, vol.71, issue.13, pp.4640-52, 2011.

T. Miyazaki, Calpain-6 confers atherogenicity to macrophages by dysregulating premRNA splicing, The Journal of clinical investigation, vol.126, issue.9, pp.3417-3449, 2016.

D. R. Pattabiraman and R. A. Weinberg, Tackling the cancer stem cells ? what challenges do they pose?, Nature Reviews Drug Discovery, vol.13, issue.7, pp.497-512, 2014.

R. Petruzzelli, HIF-2? Regulates NANOG Expression in Human Embryonic Stem Cells following Hypoxia and Reoxygenation through the Interaction with an Oct-Sox Cis Regulatory Element, PLoS ONE, vol.9, issue.10, p.108309, 2014.

X. Ran, P. Zhou, and K. Zhang, Autophagy plays an important role in stemness mediation and the novel dual function of EIG121 in both autophagy and stemness regulation of endometrial carcinoma JEC cells, International Journal of Oncology, vol.51, issue.2, pp.644-656, 2017.

S. B. Rho, Calpain 6 supports tumorigenesis by inhibiting apoptosis and facilitating angiogenesis, Cancer letters, vol.271, issue.2, pp.306-319, 2008.

N. E. Sharpless and R. A. Depinho, Cancer: Crime and punishment, Nature, vol.436, issue.7051, pp.636-637, 2005.

M. Suva, Identification of Cancer Stem Cells in Ewing's Sarcoma, Cancer Research, vol.69, issue.5, pp.1776-1781, 2009.

K. Tonami, Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1, Journal of cell science, vol.124, pp.1214-1237, 2011.

K. Tonami, Calpain-6 Deficiency Promotes Skeletal Muscle Development and Regeneration, PLoS Genetics, vol.9, issue.8, pp.1-11, 2013.

K. Tonami, Calpain-6 deficiency promotes skeletal muscle development and regeneration, PLoS genetics, vol.9, issue.8, p.1003668, 2013.

K. Tonami, Calpain 6 is involved in microtubule stabilization and cytoskeletal organization, Molecular and cellular biology, vol.27, issue.7, pp.2548-61, 2007.

P. Valent, Cancer stem cell definitions and terminology: the devil is in the details, Nature Reviews Cancer, vol.12, issue.11, pp.767-775, 2012.

Y. Wang, Endogenous miRNA Sponge lincRNA-RoR Regulates Oct4, Nanog, and Sox2 in Human Embryonic Stem Cell Self-Renewal, Developmental Cell, vol.25, issue.1, pp.69-80, 2013.

Y. Yang, Autophagy regulates the stemness of cervical cancer stem cells, Biologics: Targets and Therapy, vol.11, pp.71-79, 2017.

S. K. Zaidi, Bivalent Epigenetic Control of Oncofetal Gene Expression in Cancer, Molecular and Cellular Biology, pp.352-369, 2017.

D. M. Adelman, E. Maltepe, and M. C. Simon, Multilineage embryonic hematopoiesis requires hypoxic ARNT activity, Genes and Development, vol.13, issue.19, pp.2478-2483, 1999.

A. S. Adhikari, N. Agarwal, B. M. Wood, C. Porretta, B. Ruiz et al., CD117 and Stro-1 identify osteosarcoma tumor-initiating cells associated with metastasis and drug resistance, Cancer Research, vol.70, issue.11, pp.4602-4614, 2010.

M. Al-hajj, M. S. Wicha, A. Benito-hernandez, S. J. Morrison, and M. F. Clarke, Prospective identification of tumorigenic breast cancer cells, Proc Natl Acad Sci, vol.100, 2003.

D. Alava, C. R. Antonescu, A. Panizo, D. Leung, P. A. Meyers et al., Pronostic impact of P53 status in Ewing sarcoma, Cancer, issue.89, pp.783-792, 2000.

A. Alfranca, L. Martinez-cruzado, J. Tornin, A. Abarrategi, T. Amaral et al., Bone microenvironment signals in osteosarcoma development, Cellular and Molecular Life Sciences, vol.72, issue.16, pp.3097-3113, 2015.


C. Allemani, H. K. Weir, H. Carreira, R. Harewood, D. Spika et al., , vol.385, pp.62038-62047, 2016.

M. Allen and J. L. Jones, Jekyll and Hyde: The role of the microenvironment on the progression of cancer, Journal of Pathology, vol.223, issue.2, pp.162-176, 2011.


M. F. Amary, K. Bacsi, F. Maggiani, S. Damato, D. Halai et al., IDH1 and IDH2 mutations are frequent events in central chondrosarcoma and central and periosteal chondromas but not in other mesenchymal tumours, Journal of Pathology, vol.224, issue.3, pp.334-343, 2011.

C. R. Antonescu, G. Wl, and M. S. Magid, Molecular variants of EWS-WT1 gene fusion in desmoplastic small round cell tumor, Diagn Mol Pathol, issue.7, pp.24-28, 1998.

P. M. Aponte and A. Caicedo, Stemness in cancer: Stem cells, cancer stem cells, and their microenvironment, Stem Cells International, 2017.

L. Armstrong, Epigenetic Control of Embryonic Stem Cell Differentiation, Stem Cell Reviews and Reports, vol.8, issue.1, pp.67-77, 2012.

O. Awad, J. T. Yustein, P. Shah, N. Gul, V. Katuri et al., High ALDH activity identifies chemotherapy-resistant Ewing's sarcoma stem cells that retain sensitivity to EWS-Fli1 inhibition, PLoS ONE, issue.11, p.5, 2010.


J. Bajaj, B. Zimdahl, and T. Reya, Fearful symmetry: Subversion of asymmetric division in cancer development and progression, Cancer Research, vol.75, issue.5, pp.792-797, 2015.

N. J. Balamuth and R. B. Womer, Ewing's sarcoma, The Lancet Oncology, vol.11, issue.2, pp.184-192, 2010.

D. Balicki, Moving Forward in Human Mammary Stem Cell Biology and Breast Cancer Prognostication Using ALDH1, Cell Stem Cell, vol.1, issue.5, pp.485-487, 2007.


A. J. Bannister and T. Kouzarides, Regulation of chromatin by histone modifications, Cell Research, vol.21, issue.3, pp.381-395, 2011.

I. Ben-porath, M. Thomson, V. Carey, R. Ge, G. Bell et al., An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors, Nature Genetics, vol.40, issue.5, pp.499-507, 2008.

M. Berika, M. E. Elgayyar, and A. H. El-hashash, Asymmetric cell division of stem cells in the lung and other systems, Frontiers in Cell and Developmental Biology, vol.2, p.33, 2014.

M. Bi, C. Naczki, M. Koritzinsky, D. Fels, J. Blais et al., ER stressregulated translation increases tolerance to extreme hypoxia and promotes tumor growth, The EMBO Journal, vol.24, issue.19, pp.3470-3481, 2005.


S. S. Bielack, M. Werner, P. U. Tunn, K. Helmke, H. Jürgens et al., Methotrexate, doxorubicin, and cisplatin (MAP) plus maintenance pegylated interferon alfa-2b versus MAP alone in patients with resectable high-grade osteosarcoma and good histologic response to preoperative MAP: First results of the EURAMOS-1 good respons, Journal of Clinical Oncology, vol.33, issue.20, pp.2279-2287, 2015.

C. Blattmann, M. Thiemann, A. Stenzinger, E. K. Roth, A. Dittmar et al., Establishment of a patient-derived orthotopic osteosarcoma mouse model, Journal of Translational Medicine, vol.13, issue.1, p.136, 2015.

P. Borst, R. Evers, M. Kool, and J. Wijnholds, A family of drug transporters: the multidrug resistance-associated proteins, Journal of the National Cancer Institute, vol.92, issue.16, pp.1295-1302, 2000.

C. Bouvier, N. Macagno, Q. Nguyen, A. Loundou, C. Jiguet-jiglaire et al., Prognostic value of the Hippo pathway transcriptional coactivators YAP/TAZ and beta 1-integrin in conventional osteosarcoma, Oncotarget, vol.7, issue.40, pp.64702-64710, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01482390

H. K. Brown, M. Tellez-gabriel, and D. Heymann, Cancer stem cells in osteosarcoma, Cancer Letters, vol.386, pp.189-195, 2017.
URL : https://hal.archives-ouvertes.fr/inserm-01466078

S. A. Brown, G. A. Clines, and G. T. , Local effects of malignancy on bone, Curr Opin Endocrinol Diabetes Obes, 2007.

J. Cahu, S. Bustany, and B. Sola, Senescence-associated secretory phenotype favors the emergence of cancer stem-like cells, Cell Death & Disease, vol.3, issue.12, 2012.

, Genomic and Epigenomic Landscapes of Adult De Novo Acute Myeloid Leukemia, Cancer Genome Atlas Research Network, vol.368, issue.22, pp.2059-2074, 2013.

Y. Castillero-trejo, S. Eliazer, L. Xiang, J. A. Richardson, and R. L. Ilaria, Expression of the EWS/FLI-1 oncogene in murine primary bone-derived cells results in EWS/FLI-1-dependent, Ewing sarcoma-like tumors, Cancer Research, vol.65, issue.19, pp.8698-8705, 2005.

J. S. Castresana, C. Barrios, L. Gómez, and K. A. , Amplification of the c-myc proto-oncogene in human chondrosarcoma, Diagn Mol Pathol, 1992.

A. O. Cavazzana, J. S. Miser, J. Jefferson, and T. J. Triche, Experimental evidence for a neural origin of Ewing's sarcoma of bone, The American Journal of Pathology, vol.127, issue.3, pp.507-525, 1987.

I. Chambers and S. R. Tomlinson, The transcriptional foundation of pluripotency, Development, issue.14, pp.2311-2333, 2009.


E. Charafe-jauffret, C. Ginestier, F. Bertucci, O. Cabaud, J. Wicinski et al., ALDH1-positive cancer stem cells predict engraftment of primary breast tumors and are governed by a common stem cell program, Cancer Research, vol.73, issue.24, pp.7290-7300, 2013.
URL : https://hal.archives-ouvertes.fr/hal-01431937

E. Charafe-jauffret, C. Ginestier, F. , F. I. Tarpin, C. Diebel et al., ALDH1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer, Clinical Cancer Research, vol.16, issue.1, pp.45-55, 2010.

X. Chen, F. Fang, Y. Liou, and H. Ng, Zfp143 Regulates Nanog Through Modulation of Oct4 Binding, Stem Cells, vol.26, issue.11, pp.2759-2767, 2008.


C. Andrique,

T. Chiba, K. Kita, Y. W. Zheng, O. Yokosuka, H. Saisho et al., Side population purified from hepatocellular carcinoma cells harbors cancer stem celllike properties, Hepatology, vol.44, issue.1, pp.240-251, 2006.

S. W. Choy and S. H. Cheng, Hedgehog Signaling. Vitamins and Hormones, 2012.


Q. S. Chu, A. Mahipal, M. Schuler, F. G. De-braud, L. Dirix et al., 445Odose-Escalation Study of Sonidegib (Lde225) Plus Buparlisib (Bkm120) in Patients (Pts) With Advanced Solid Tumors, Annals of Oncology, vol.25, issue.suppl_4, pp.147-148, 2014.

J. Coppé, C. K. Patil, F. Rodier, Y. Sun, D. P. Muñoz et al., Senescence-Associated Secretory Phenotypes Reveal Cell-Nonautonomous Functions of Oncogenic RAS and the p53 Tumor Suppressor, PLoS Biology, vol.6, issue.12, 2008.

S. Cornaz-buros, N. Riggi, C. Devito, A. Sarre, I. Letovanec et al., Targeting cancer stem-like cells as an approach to defeating cellular heterogeneity in Ewing sarcoma, Cancer Research, vol.74, issue.22, pp.6610-6622, 2014.

F. Crea, E. Paolicchi, V. E. Marquez, and R. Danesi, Polycomb genes and cancer: Time for clinical application?, Critical Reviews in Oncology, vol.83, issue.2, pp.184-193, 2012.

D. E. Croall and K. Ersfeld, The calpains: modular designs and functional diversity, Genome Biology, vol.8, issue.6, 2007.

N. Dear, K. Matena, M. Vingron, and T. Boehm, A New Subfamily of Vertebrate Calpains Lacking a Calmodulin-Like Domain : Implications for Calpain Regulation and Evolution, vol.184, pp.175-184, 1997.

T. N. Dear and T. Boehm, Diverse mRNA expression patterns of the mouse calpain genes Capn5, Capn6 and Capn11 during development, Mechanisms of Development, vol.89, issue.1-2, pp.201-209, 1999.

J. Dekker, Capturing Chromosome Conformation, Science, vol.295, issue.5558, pp.1306-1311, 2002.

C. Dierks, R. Beigi, G. R. Guo, K. Zirlik, M. R. Stegert et al., Expansion of Bcr-Abl-Positive Leukemic Stem Cells Is Dependent on Hedgehog Pathway Activation, Cancer Cell, vol.14, issue.3, pp.238-249, 2008.

J. Douville, R. Beaulieu, and D. Balicki, ALDH1 as a Functional Marker of Cancer Stem and Progenitor Cells, Stem Cells and Development, vol.18, issue.1, pp.17-26, 2009.

T. Eggert, K. Wolter, J. Ji, C. Ma, T. Yevsa et al., Distinct Functions of Senescence-Associated Immune Responses in Liver Tumor Surveillance and Tumor Progression, Cancer Cell, vol.30, issue.4, pp.533-547, 2016.

C. Andrique,


C. A. Eide and B. J. Druker, Understanding cancer from the stem cells up, Nature Medicine, vol.23, issue.6, pp.656-657, 2017.

A. H. El-hashash and D. Warburton, Numb expression and asymmetric versus symmetric cell division in distal embryonic lung epithelium, The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, vol.60, issue.9, pp.675-82, 2012.

J. Erenpreisa and M. S. Cragg, Three steps to the immortality of cancer cells: senescence, polyploidy and self-renewal, Cancer Cell International, vol.13, issue.1, 2013.

. Ewing-james, Diffuse endothelioma of bone, Proc New York Path, vol.21, pp.17-24, 1921.

X. Fan, W. Matsui, L. Khaki, D. Stearns, J. Chun et al., Notch pathway inhibition depletes stem-like cells and blocks engraftment in embryonal brain tumors, Cancer Research, vol.66, issue.15, pp.7445-7452, 2006.

G. Farnie and C. R. , Mammary stem cells and breast cancer--role of Notch signalling, Stem Cell Rev, 2007.

M. Felx, M. Guyot, M. Isler, R. E. Turcotte, J. Doyon et al., Endothelin-1 (ET-1) promotes MMP-2 and MMP-9 induction involving the transcription factor NF-kappaB in human osteosarcoma, Clinical Science, vol.110, issue.6, pp.645-54, 1979.

K. Fizazi, J. Yang, S. Peleg, C. R. Sikes, E. L. Kreimann et al., , 2003.

, Prostate Cancer Cells-Osteoblast Interaction Shifts Expression of Growth / Survivalrelated Genes in Prostate Cancer and Reduces Expression of Osteoprotegerin in Osteoblasts Prostate Cancer Cells-Osteoblast Interaction Shifts Expression of Growth / Surviv, vol.9, pp.2587-2597

. Fletcher;-unni-;-mertens, World Health Organization Classification of Tumours Pathology and Genetics of Tumours of Soft Tissue and Bone, vol.177, pp.1365-76, 2002.

H. Folkerts, S. Hilgendorf, A. T. Wierenga, J. Jaques, A. B. Mulder et al., Inhibition of autophagy as a treatment strategy for p53 wild-type acute myeloid leukemia, Cell Death and Disease, vol.8, issue.7, 2017.

A. Franchi, A. Calzolari, and Z. G. , Immunohistochemical detection of c-fos and c-jun expression in osseous and cartilaginous tumours of the skeleton, Virchows Archiv, 1998.

T. Fu, I. Hsieh, J. Cheng, M. Tsai, Y. Hou et al., Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, vol.45, pp.89-95, 2016.

T. Fujiwara and T. Ozaki, Overcoming Therapeutic Resistance of Bone Sarcomas: Overview of the Molecular Mechanisms and Therapeutic Targets for Bone Sarcoma Stem Cells, Stem Cells International, 2016.

L. García-prat, M. Martínez-vicente, E. Perdiguero, L. Ortet, J. Rodríguez-ubreva et al., Autophagy maintains stemness by preventing senescence, Nature, vol.529, issue.7584, pp.37-42, 2016.

H. Gelderblom, P. C. Hogendoorn, S. D. Dijkstra, C. S. Van-rijswijk, A. D. Krol et al., The Clinical Approach Towards Chondrosarcoma LEARNING OBJECTIVES, pp.320-329, 2016.

C. P. Gibbs, V. G. Kukekov, J. D. Reith, O. Tchigrinova, O. N. Suslov et al., Stem-like cells in bone sarcomas: implications for tumorigenesis, Neoplasia, vol.7, issue.11, pp.967-76, 2005.


C. Ginestier, M. H. Hur, E. Charafe-jauffret, F. Monville, J. Dutcher et al., ALDH1 Is a Marker of Normal and Malignant Human Mammary Stem Cells and a Predictor of Poor Clinical Outcome, Cell Stem Cell, vol.1, issue.5, pp.555-567, 2007.
URL : https://hal.archives-ouvertes.fr/hal-01431968

M. E. Gonzalez, H. M. Moore, X. Li, K. A. Toy, W. Huang et al., EZH2 expands breast stem cells through activation of NOTCH1 signaling, Proceedings of the National Academy of Sciences, vol.111, issue.8, pp.3098-3103, 2014.


E. Gras, E. Belaidi, A. Briançon-marjollet, J. Pépin, C. Arnaud et al., Endothelin-1 mediates intermittent hypoxia-induced inflammatory vascular remodeling through HIF-1 activation, Journal of Applied Physiology, vol.120, issue.4, pp.437-443, 2016.

M. V. Guijarro, S. C. Ghivizzani, and C. P. Gibbs, Animal Models in Osteosarcoma, Frontiers in Oncology, vol.4, pp.1-7, 2014.

M. V. Gustafsson, X. Zheng, T. Pereira, K. Gradin, S. Jin et al., Hypoxia requires Notch signaling to maintain the undifferentiated cell state, Developmental Cell, vol.9, issue.5, pp.617-628, 2005.

J. M. Gwak, M. Kim, H. J. Kim, M. H. Jang, and S. Y. Park, Expression of embryonal stem cell transcription factors in breast cancer: Oct4 as an indicator for poor clinical outcome and tamoxifen resistance, Oncotarget, vol.8, issue.22, pp.36305-36318, 2017.


D. Hanahan and R. A. Weinberg, Hallmarks of cancer: The next generation, Cell, vol.144, issue.5, pp.646-674, 2011.

N. Haraguchi, T. Utsunomiya, H. Inoue, F. Tanaka, K. Mimori et al., Characterization of a side population of cancer cells from human gastrointestinal system, Stem Cells, vol.24, issue.3, pp.506-513, 2006.


C. Hirschmann-jax, A. E. Foster, G. G. Wulf, J. G. Nuchtern, T. W. Jax et al., A distinct "side population" of cells with high drug efflux capacity in human tumor cells, Proceedings of the National Academy of Sciences of the United States of America, vol.101, issue.39, pp.14228-14261, 2004.

M. M. Ho, A. V. Ng, S. Lam, and J. Y. Hung, Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells, Cancer Research, vol.67, issue.10, pp.4827-4833, 2007.

T. Hoey, W. C. Yen, F. Axelrod, J. Basi, L. Donigian et al., DLL4 blockade inhibits tumor growth and reduces tumor-initiating cell frequency, Cell Stem Cell, 2009.

J. M. Hong, S. L. Teitelbaum, T. Kim, F. P. Ross, S. Kim et al., Calpain-6, a target molecule of glucocorticoids, regulates osteoclastic bone resorption via cytoskeletal organization and microtubule acetylation, Journal of Bone and Mineral Research : The Official Journal of the American Society for Bone and Mineral Research, vol.26, issue.3, pp.657-65, 2011.

K. Honoki, H. Fujii, A. Kubo, A. Kido, T. Mori et al., Possible involvement of stem-like populations with elevated ALDH1 in sarcomas for chemotherapeutic drug resistance, pp.501-505, 2010.

K. Ida, EWS-FLI1 and EWS-ERG chimeric mRNAs in Ewing's sarcoma and primitive neuroectodermal tumor, p.100, 1995.

I. Ifergan, I. Meller, J. Issakov, and Y. G. Assaraf, Reduced Folate Carrier Protein Expression in Osteosarcoma: Implications for the Prediction of Tumor Chemosensitivity, Cancer, vol.98, issue.9, pp.1958-1966, 2003.

H. Iida, M. Suzuki, R. Goitsuka, and H. Ueno, Hypoxia induces CD133 expression in human lung cancer cells by up-regulation of OCT3/4 and SOX2, International Journal of Oncology, vol.40, issue.1, pp.71-79, 2012.

O. Iriondo, M. Rábano, G. Domenici, O. Carlevaris, J. A. López-ruiz et al., Distinct breast cancer stem/progenitor cell populations require either HIF1? or loss of PHD3 to expand under hypoxic conditions, Oncotarget, vol.6, issue.31, pp.31721-31760, 2015.

N. Jamil, S. Howie, and D. M. Salter, Therapeutic molecular targets in human chondrosarcoma, International Journal of Experimental Pathology, vol.91, issue.5, pp.387-393, 2010.

L. Kager, A. Zoubek, M. Dominkus, S. Lang, N. Bodmer et al., , 2010.

C. Andrique,

, Osteosarcoma in very young children, Cancer, vol.116, issue.22, pp.5316-5324

T. Kang, T. Yevsa, N. Woller, L. Hoenicke, T. Wuestefeld et al., Senescence surveillance of pre-malignant hepatocytes limits liver cancer development, Nature, vol.479, issue.7374, pp.547-551, 2011.

M. Kansara, M. W. Teng, M. J. Smyth, and D. M. Thomas, Translational biology of osteosarcoma, vol.14, pp.722-735, 2014.


J. Kaur and J. Debnath, Autophagy at the crossroads of catabolism and anabolism, Nature Reviews Molecular Cell Biology, vol.16, issue.8, pp.461-472, 2015.


C. M. Kenific, S. J. Stehbens, J. Goldsmith, A. M. Leidal, N. Faure et al., NBR 1 enables autophagy-dependent focal adhesion turnover, Journal of Cell Biology, vol.212, issue.5, pp.577-590, 2016.

J. A. Knoblich, Asymmetric cell division: recent developments and their implications for tumour biology, Nature Reviews. Molecular Cell Biology, vol.11, issue.12, pp.849-860, 2010.

A. H. Ko, N. Loconte, M. A. Tempero, E. J. Walker, K. Kelley et al., A Phase I Study of FOLFIRINOX Plus IPI-926, a Hedgehog Pathway Inhibitor, for Advanced Pancreatic Adenocarcinoma, Pancreas, issue.0, pp.1-6, 2015.


T. Kondo, T. Setoguchi, and T. Taga, Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line, Proceedings of the National Academy of Sciences of the United States of America, vol.101, issue.3, pp.781-787, 2004.


A. Koong, K. Che, and A. Giaccia, Hypoxia causes the activation of nuclear factor kappa through the phosphorilation of I-kB on tyrosine residues, Cancer Res, issue.54, pp.1425-1430, 1994.

H. Kovar and . Bernard-alain, CD99-Positive "'Ewing's Sarcoma"' from Mouse Bone Marrow-Derived Mesenchymal Progenitor Cells?, Cancer Research, vol.66, issue.19, pp.9786-9787, 2006.

A. Kreso and J. E. Dick, Evolution of the cancer stem cell model, Cell Stem Cell, vol.14, issue.3, pp.275-291, 2014.

L. Lacerda, L. Pusztai, and W. A. Woodward, The role of tumor initiating cells in drug resistance of breast cancer: Implications for future therapeutic approaches, Drug Resistance Updates, vol.13, issue.4-5, pp.99-108, 2010.

F. Lamoureux, G. Moriceau, G. Picarda, J. Rousseau, V. Trichet et al., Regulation of osteoprotegerin pro-or anti-tumoral activity by bone tumor microenvironment, Biochimica et Biophysica Acta -Reviews on Cancer, issue.1, pp.17-24, 2010.

C. Andrique,

T. Lapidot, C. Sirard, J. Vormoor, B. Murdoch, T. Hoang et al., A cell initiating human acute myeloid leukaemia after transplantation into SCID mice, Nature, vol.367, 1994.

E. R. Lawlor, J. F. Lim, W. Tao, C. Poremba, C. J. Chow et al., The Ewing tumor family of peripheral primitive neuroectodermal tumors expresses human gastrin-releasing peptide, Cancer Research, vol.58, issue.11, pp.2469-2476, 1998.

N. Lee, D. Kim, D. Kim, and D. Kim, Cancer Metabolism: Fueling More than Just Growth, Molecules and Cells, vol.39, issue.12, pp.847-854, 2016.


S. J. Lee, B. G. Kim, Y. Choi, . La, and J. W. Lee, Increased expression of calpain 6 during the progression of uterine cervical neoplasia: Immunohistochemical analysis, Oncology Reports, vol.19, issue.4, pp.859-863, 2008.

Y. H. Lee, H. W. Yang, L. C. Yang, M. Y. Lu, L. L. Tsai et al., DHFR and MDR1 upregulation is associated with chemoresistance in osteosarcoma stem-like cells, Oncology Letters, vol.14, issue.1, pp.171-179, 2017.

E. Letouzé, C. Martinelli, C. Loriot, N. Burnichon, N. Abermil et al., SDH Mutations Establish a Hypermethylator Phenotype in Paraganglioma, Cancer Cell, vol.23, issue.6, pp.739-752, 2013.

G. D. Letson and C. A. Muro-cacho, Genetic and molecular abnormalities in tumors of the bone and soft tissues, Cancer Control, vol.8, issue.3, pp.239-251, 2001.

E. Li, Chromatin modification and epigenetic reprogramming in mammalian development, Nature Reviews Genetics, vol.3, issue.9, pp.662-673, 2002.

J. Liang, M. Wan, Y. Zhang, P. Gu, H. Xin et al., Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells, Nature Cell Biology, vol.10, issue.6, pp.731-739, 2008.

F. Liao, H. Xu, N. Torrey, P. Road, and L. Jolla, Navigating the bone marrow niche: translational insights and cancer-driven dysfunction, Nature Reviews Rheumatol, vol.2, issue.74, pp.154-168, 2015.

R. K. Limin and Z. Dennis, Bone Sarcomas in Pediatrics : Progress in Our Understanding of Tumor Biology and Implications for Therapy, Pediatric Drugs, vol.17, issue.4, pp.257-271, 2015.

P. P. Lin, M. K. Pandey, F. Jin, A. K. Raymond, H. Akiyama et al., Targeted mutation of p53 and Rb in mesenchymal cells of the limb bud produces sarcomas in mice, Carcinogenesis, vol.30, issue.10, pp.1789-1795, 2009.

Y. Liu, C. Mei, L. Sun, X. Li, M. Liu et al., The PI3K-Akt pathway regulates calpain 6 expression, proliferation, and apoptosis, Cellular Signalling, vol.23, issue.5, pp.827-836, 2011.

C. Andrique,

Y. Liu, Y. Wang, X. Sun, C. Mei, L. Wang et al., miR-449a promotes liver cancer cell apoptosis by down regulation of Calpain 6 and, vol.2, pp.13491-13501, 2015.

R. Lu, P. Wang, T. Parton, Y. Zhou, K. Chrysovergis et al., Epigenetic Perturbations by Arg882-Mutated DNMT3A Potentiate Aberrant Stem Cell Gene-Expression Program and Acute Leukemia Development, Cancer Cell, vol.30, issue.1, pp.92-107, 2016.

L. Leloup and A. W. , Calpains as potential anti-cancer targets, Expert Opin Ther Targets, vol.119, pp.5124-5136, 2010.
URL : https://hal.archives-ouvertes.fr/hal-01740915

P. Marcato, C. A. Dean, C. A. Giacomantonio, and P. W. Lee, Aldehyde dehydrogenase its role as a cancer stem cell marker comes down to the specific isoform, Cell Cycle, vol.10, issue.9, pp.1378-1384, 2011.

A. Marion, F. X. Dieudonné, A. Patiño-garcia, F. Lecanda, P. J. Marie et al., Calpain-6 is an endothelin-1 signaling dependent protective factor in chemoresistant osteosarcoma, International Journal of Cancer, vol.130, issue.11, pp.2514-2525, 2012.

K. Markiewicz, K. Zeman, A. Kozar, and G. M. , Evaluation of selected cytokines in children and adolescents with osteosarcoma at diagnosis -preliminary report, Med Wieku Rozwoj, 2011.

S. R. Martins-neves, Á. O. Lopes, A. Do-carmo, A. A. Paiva, P. C. Simões et al., Therapeutic implications of an enriched cancer stem-like cell population in a human osteosarcoma cell line, BMC Cancer, vol.12, issue.1, p.139, 2012.

J. Mathieu, Z. Zhang, W. Zhou, A. J. Wang, J. M. Heddleston et al., HIF induces human embryonic stem cell markers in cancer cells, Cancer Research, vol.71, issue.13, pp.4640-52, 2011.

A. Mayle, L. Yang, B. Rodriguez, T. Zhou, E. Chang et al., Dnmt3a loss predisposes murine hematopoietic stem cells to malignant transformation, vol.125, pp.629-639, 2015.

B. B. Mcconnell and V. W. Yang, Mammalian Krueppel-Like Factors in Health and Diseases, Physiological Reviews, vol.90, 2010.


P. Mehlen and A. Puisieux, Metastasis: a question of life or death, Nature Reviews Cancer, vol.6, issue.6, pp.449-458, 2006.

A. Merchant, Targeting Hedgehog -a Cancer Stem Cell Pathway, Clin Cancer Res, vol.86, issue.12, pp.3279-3288, 2010.

B. J. Miller, Y. Gao, and K. R. Duchman, Does surgery or radiation provide the best overall survival in Ewing's sarcoma? A review of the National Cancer Data Base, Journal of Surgical Oncology, vol.116, issue.3, pp.384-390, 2017.

N. Mitsutake, A. Iwao, K. Nagai, H. Namba, A. Ohtsuru et al., Characterization of side population in thyroid cancer cell lines: Cancer stem-like cells are enriched partly but not exclusively, Endocrinology, vol.148, issue.4, pp.1797-1803, 2007.

T. Miyazaki, K. Tonami, S. Hata, T. Aiuchi, K. Ohnishi et al., , 2016.

, Calpain-6 confers atherogenicity to macrophages by dysregulating pre-mRNA splicing, vol.126, pp.3417-3432

K. Miyazawa, T. Tanaka, D. Nakai, N. Morita, and K. Suzuki, Immunohistochemical expression of four different stem cell markers in prostate cancer: High expression of NANOG in conjunction with hypoxia-inducible factor-1?? expression is involved in prostate epithelial malignancy, Oncology Letters, vol.8, issue.3, pp.985-992, 2014.


K. Moitra, H. Lou, and M. Dean, Multidrug Efflux Pumps and Cancer Stem Cells: Insights Into Multidrug Resistance and Therapeutic Development, Clinical Pharmacology & Therapeutics, vol.89, issue.4, pp.491-502, 2011.

J. B. Moore, D. M. Loeb, K. U. Hong, P. H. Sorensen, T. J. Triche et al., Epigenetic reprogramming and re-differentiation of a Ewing sarcoma cell line, Frontiers in Cell and Developmental Biology, vol.3, p.15, 2015.

D. Muñoz-espín and M. Serrano, Cellular senescence: from physiology to pathology, Nature Reviews Molecular Cell Biology, vol.15, issue.7, pp.482-496, 2014.


M. Murar and A. Vaidya, Cancer stem cell markers: premises and prospects, Biomarkers in Medicine, vol.9, issue.12, pp.1331-1373, 2015.

A. J. Mutsaers and C. R. Walkley, Cells of origin in osteosarcoma: Mesenchymal stem cells or osteoblast committed cells?, Bone, vol.62, pp.56-63, 2014.


H. M. Namløs, S. H. Kresse, C. R. Müller, J. Henriksen, R. Holdhus et al., Global gene expression profiling of human osteosarcomas reveals metastasisassociated chemokine pattern, Sarcoma, 2012.

V. Neumeister and D. Rimm, Is ALDH1 a good method for definition of breast cancer stem cells?, Breast Cancer Research and Treatment, vol.123, issue.1, pp.109-111, 2010.

C. A. O'brien, A. Pollett, S. Gallinger, and J. E. Dick, A human colon cancer cell capable of initiating tumour growth in immunodeficient mice, Nature, vol.445, issue.7123, pp.106-110, 2007.

C. Andrique,

K. P. Olive, M. Jacobetz, C. J. Davidson, D. Mcintyre, D. Honess et al., Chemotherapy in a Mouse Model of Pancreatic Cancer, Cancer Research, vol.324, issue.5933, pp.1457-1461, 2010.

M. Onda, S. Matsuda, S. Higaki, T. Iijima, J. I. Fukushima et al., ErbB-2 expression is correlated with poor prognosis for patients with osteosarcoma, Cancer, vol.77, issue.1, pp.71-78, 1996.

A. Pagotto, G. Pilotto, E. L. Mazzoldi, M. O. Nicoletto, S. Frezzini et al., Autophagy inhibition reduces chemoresistance and tumorigenic potential of human ovarian cancer stem cells, Cell Death and Disease, vol.8, issue.7, 2017.

D. R. Pattabiraman and R. A. Weinberg, Tackling the cancer stem cells -what challenges do they pose?, Nature Reviews Drug Discovery, vol.13, issue.7, pp.497-512, 2014.

S. Piperno-neumann, M. C. Le-deley, F. Rédini, H. Pacquement, P. Marec-bérard et al., Zoledronate in combination with chemotherapy and surgery to treat osteosarcoma (OS2006): a randomised, multicentre, open-label, phase 3 trial, The Lancet Oncology, vol.17, issue.8, pp.30096-30097, 2016.
URL : https://hal.archives-ouvertes.fr/inserm-01702092

D. F. Poulson, Chromosomal Deficiencies and the Embryonic Development of Drosophila Melanogaster, Proceedings of the National Academy of Sciences of the United States of America, vol.23, issue.3, pp.133-137, 1937.

P. Prasad, S. A. Mittal, J. Chongtham, S. Mohanty, and T. Srivastava, HypoxiaMediated Epigenetic Regulation of Stemness in Brain Tumor Cells, Stem Cells, vol.35, issue.6, pp.1468-1478, 2017.

S. S. Prime, N. Cirillo, Y. Hassona, D. W. Lambert, I. C. Paterson et al., Fibroblast activation and senescence in oral cancer, Journal of Oral Pathology & Medicine, 2016.

R. Rajaraman, M. M. Rajaraman, and S. R. Rajaraman, Neosis e a paradigm of selfrenewal in cancer, vol.29, pp.1084-1097, 2005.

E. Resetkova, J. S. Reis-filho, R. K. Jain, R. Mehta, M. A. Thorat et al., Prognostic impact of ALDH1 in breast cancer: A story of stem cells and tumor microenvironment, Breast Cancer Research and Treatment, vol.123, issue.1, pp.97-108, 2010.

S. B. Rho, H. J. Byun, S. Y. Park, and T. Chun, Calpain 6 supports tumorigenesis by inhibiting apoptosis and facilitating angiogenesis, Cancer Letters, vol.271, issue.2, pp.306-313, 2008.

C. Andrique,

L. Ricci-vitiani, D. G. Lombardi, E. Pilozzi, M. Biffoni, M. Todaro et al., Identification and expansion of human colon-cancer-initiating cells, Nature, p.445, 2007.

N. Riggi, L. Cironi, P. Provero, M. Suva, K. Kaloulis et al., Development of Ewing's Sarcoma from Primary Bone MarrowDerived Mesenchymal Progenitor Cells, Cancer Res, vol.65, issue.24, pp.11459-11468, 2005.

N. Riggi and I. Stamenkovic, The Biology of Ewing sarcoma, Cancer Letters, vol.254, issue.1, pp.1-10, 2007.

N. Riggi, M. L. Suvà, D. Suvà, L. Cironi, P. Provero et al., EWS-FLI-1 expression triggers a ewing's sarcoma initiation program in primary human mesenchymal stem cells, Cancer Research, vol.68, issue.7, pp.2176-2185, 2008.


A. Riggs and T. Porter, Overview of epigenetic mechanisms, Epigenetic mechanisms of gene regulation, 1996.

R. Rubio, I. Gutierrez-aranda, A. I. Sáez-castillo, A. Labarga, M. Rosu-myles et al., The differentiation stage of p53-Rb-deficient bone marrow mesenchymal stem cells imposes the phenotype of in vivo sarcoma development, Oncogene, vol.32, issue.41, pp.4970-4980, 2013.

S. Salas, C. Jiguet-jiglaire, L. Campion, C. Bartoli, F. Frassineti et al., Correlation between ERK1 and STAT3 expression and chemoresistance in patients with conventional osteosarcoma, BMC Cancer, vol.14, p.606, 2014.

A. M. Samuel, J. Costa, and D. M. Lindskog, Genetic alterations in chondrosarcomasKeys to targeted therapies?, Cellular Oncology, vol.37, issue.2, pp.95-105, 2014.


R. Scheilf, DNA looping, Annu Rev Biochem, vol.61, pp.199-223, 1992.


A. H. Schinkel, J. J. Smit, O. Van-tellingen, J. H. Beijnen, E. Wagenaar et al., Disruption of the mouse mdr1a P-glycoprotein gene leads to a deficiency in the blood-brain barrier and to increased sensitivity to drugs, Cell, vol.77, issue.4, pp.90212-90219, 1994.

A. N. Schuetz, B. P. Rubin, J. R. Goldblum, B. Shehata, S. W. Weiss et al., Intercellular junctions in Ewing sarcoma/primitive neuroectodermal tumor: additional evidence of epithelial differentiation, Modern Pathology : An Official Journal of the United States and Canadian Academy of Pathology, Inc, vol.18, issue.11, pp.1403-1413, 2005.

C. Andrique,

A. Schulenburg, K. Br??mswig, H. Herrmann, H. Karlic, I. Mirkina et al., Neoplastic stem cells: Current concepts and clinical perspectives, Critical Reviews in Oncology, vol.76, issue.2, pp.79-98, 2010.


N. C. Sheffield, G. Pierron, J. Klughammer, P. Datlinger, A. Schönegger et al., DNA methylation heterogeneity defines a disease spectrum in Ewing sarcoma, Nature Medicine, vol.23, issue.3, pp.386-395, 2017.

J. K. Shen, G. M. Cote, E. Choy, P. Yang, D. Harmon et al., , vol.2, pp.690-698, 2015.

K. M. Skubitz and A. P. Skubitz, Differential gene expression in leiomyosarcoma, Cancer, vol.98, issue.5, pp.1029-1038, 2003.

A. Soeda, M. Park, D. Lee, A. Mintz, A. Androutsellis-theotokis et al., Hypoxia promotes expansion of the CD133-positive glioma stem cells through activation of HIF-1alpha, Oncogene, vol.28, issue.45, pp.3949-3959, 2009.


A. B. Stergachis, S. Neph, A. Reynolds, R. Humbert, B. Miller et al., Developmental fate and cellular maturity encoded in human regulatory DNA landscapes, Cell, vol.154, issue.4, pp.888-903, 2013.


S. J. Storr, N. O. Carragher, M. C. Frame, T. Parr, and S. G. Martin, The calpain system and cancer, Nature Reviews Cancer, vol.11, issue.5, pp.364-374, 2011.

M. L. Suvà, N. Riggi, M. Janiszewska, I. Radovanovic, P. Provero et al., EZH2 is essential for glioblastoma cancer stem cell maintenance, Cancer Research, vol.69, issue.24, pp.9211-9218, 2009.

M. L. Suva, N. Riggi, J. C. Stehle, K. Baumer, S. Tercier et al., Identification of cancer stem cells in Ewing's sarcoma, Cancer Res, vol.69, issue.5, pp.1776-1781, 2009.

P. P. Szotek, R. Pieretti-vanmarcke, P. T. Masiakos, D. M. Dinulescu, D. Connolly et al., Ovarian cancer side population defines cells with stem celllike characteristics and Mullerian Inhibiting Substance responsiveness, Proceedings of the National Academy of Sciences, vol.103, issue.30, pp.11154-11159, 2006.


H. Szutorisz, C. Canzonetta, A. Georgiou, C. Chow, L. Tora et al., Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage, Molecular and Cellular Biology, vol.25, issue.5, pp.1804-1820, 2005.
URL : https://hal.archives-ouvertes.fr/hal-00187771

C. Andrique,

N. Takebe, P. J. Harris, R. Q. Warren, and S. P. Ivy, Targeting cancer stem cells by inhibiting Wnt, Notch, and Hedgehog pathways, Nature Reviews. Clinical Oncology, vol.8, issue.2, pp.97-106, 2011.

J. Tan, Y. Yan, X. Wang, Y. Jiang, and H. E. Xu, EZH2: biology, disease, and structurebased drug discovery, Acta Pharmacologica Sinica, vol.35, issue.2, pp.161-174, 2014.

R. Tannishtha, S. J. Morrison, M. F. Clarke, and I. L. Weissman, Stem Cells, Cancer, and Cancer Stem Cells, Nature, vol.414, pp.105-111, 2001.

H. Tao, H. Li, Y. Su, D. Feng, X. Wang et al., Histone methyltransferase G9a and H3K9 dimethylation inhibit the self-renewal of glioma cancer stem cells, Molecular and Cellular Biochemistry, vol.394, issue.1, pp.23-30, 2014.

S. Taran, R. Taran, and N. Malipatil, Pediatric osteosarcoma: An updated review, Indian Journal of Medical and Paediatric Oncology, 2017.

V. Tirino, V. Desiderio, R. D'aquino, F. De-francesco, G. Pirozzi et al., Detection and characterization of CD133+ cancer stem cells in human solid tumours, PLoS One, vol.3, issue.10, 2008.

V. Tirino, V. Desiderio, F. Paino, A. De-rosa, F. Papaccio et al., Human primary bone sarcomas contain CD133+ cancer stem cells displaying high tumorigenicity in vivo, The FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol.25, issue.6, pp.2022-2030, 2011.


F. Tirode, K. Laud-duval, A. Prieur, B. Delorme, P. Charbord et al., , 2007.

, Mesenchymal Stem Cell Features of Ewing Tumors. Cancer Cell, vol.11, issue.5, pp.421-429

K. Tonami, S. Hata, K. Ojima, Y. Ono, Y. Kurihara et al., Calpain-6 Deficiency Promotes Skeletal Muscle Development and Regeneration, PLoS Genetics, vol.9, issue.8, pp.1-11, 2013.

K. Tonami, Y. Kurihara, H. Aburatani, Y. Uchijima, T. Asano et al., Calpain 6 is involved in microtubule stabilization and cytoskeletal organization, Molecular and Cellular Biology, vol.27, issue.7, pp.2548-61, 2007.

K. Tonami, Y. Kurihara, S. Arima, K. Nishiyama, Y. Uchijima et al., Cancer stem cells in osteosarcoma: Recent progress and perspective, Cancer Research, vol.7, issue.1, pp.1311-1328, 2011.

C. Andrique,

K. Tonami, Y. Kurihara, S. Arima, K. Nishiyama, Y. Uchijima et al., Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1, Journal of Cell Science, vol.124, pp.1214-1223, 2011.

P. Valent, D. Bonnet, R. Maria, . De, T. Lapidot et al., terminology : the devil is in the details, Nature Publishing Group, vol.12, issue.11, pp.767-775, 2012.

A. K. Virmani, A. Rathi, U. G. Sathyanarayana, A. Padar, C. X. Huang et al., Aberrant methylation of the adenomatous polyposis coli (APC) gene promoter 1A in breast and lung carcinomas, Clin Cancer Res, vol.7, issue.7, pp.1998-2004, 2001.

J. Wang, L. Guo, L. Chen, Y. Zeng, and S. H. Lu, Identification of Cancer Stem Cell-Like Side Population Cells in Human Nasopharyngeal Carcinoma Cell Line, Cancer Research, vol.67, issue.8, pp.3716-3724, 2007.

X. Wang, B. Zheng, R. R. Zhang, S. Li, J. J. Mulvihill et al., Notch Promotes Radioresistance of Glioma Stem Cells Jialiang, Cancer Research, vol.9, issue.3, pp.231-242, 2011.

W. Otto, The metabolism of carcinoma cells, J Cancer Res, vol.9, pp.148-163, 1925.

P. S. Ward, J. Patel, D. R. Wise, O. Abdel-wahab, B. D. Bennett et al., The Common Feature of Leukemia-Associated IDH1 and IDH2 Mutations Is a Neomorphic Enzyme Activity Converting ??-Ketoglutarate to 2-Hydroxyglutarate, Cancer Cell, vol.17, issue.3, pp.225-234, 2010.

M. Weber, I. Hellmann, M. B. Stadler, L. Ramos, S. Pääbo et al., Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome, Nature Genetics, vol.39, issue.4, pp.457-466, 2007.

S. H. Wong, D. L. Goode, M. Iwasaki, M. C. Wei, H. P. Kuo et al., The H3K4-Methyl Epigenome Regulates Leukemia Stem Cell Oncogenic Potential, Cancer Cell, vol.28, issue.2, pp.198-209, 2015.

T. L. Wong, N. Che, and S. Ma, Reprogramming of central carbon metabolism in cancer stem cells, Biochimica et Biophysica Acta, issue.7, pp.1728-1738, 2017.

C. Wu, Q. Wei, V. Utomo, P. Nadesan, H. Whetstone et al., Side population cells isolated from mesenchymal neoplasms have tumor initiating potential, Cancer Research, vol.67, issue.17, pp.8216-8222, 2007.

Y. Xiang, F. Li, L. Wang, A. Zheng, J. Zuo et al., Decreased calpain 6 expression is associated with tumorigenesis and poor prognosis in HNSCC, Oncology Letters, vol.13, issue.4, pp.2237-2243, 2017.

C. Andrique,

W. Xu, H. Yang, Y. Liu, Y. Yang, P. Wang et al., Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of ?-ketoglutarate-dependent dioxygenases, Cancer Cell, vol.19, issue.1, pp.17-30, 2011.

X. D. Xu, S. X. Shao, H. P. Jiang, Y. W. Cao, Y. H. Wang et al., Warburg effect or reverse warburg effect? a review of cancer metabolism, Oncology Research and Treatment, vol.38, issue.3, pp.117-122, 2015.

M. Yang, M. Yan, R. Zhang, J. Li, and Z. Luo, Side population cells isolated from human osteosarcoma are enriched with tumor-initiating cells, Cancer Science, vol.102, issue.10, pp.1774-1781, 2011.

M. Yang, R. Zhang, M. Yan, Z. Ye, W. Liang et al., Detection and characterization of side population in Ewing's sarcoma SK-ES-1 cells in vitro, Biochemical and Biophysical Research Communications, vol.391, issue.1, pp.1062-1066, 2010.

Y. Yang, L. Yu, J. Li, Y. H. Yuan, X. L. Wang et al., Autophagy regulates the stemness of cervical cancer stem cells, Biologics: Targets and Therapy, vol.11, pp.71-79, 2017.

S. K. Zaidi, S. E. Frietze, J. A. Gordon, J. L. Heath, T. Messier et al., , 2017.

, Bivalent Epigenetic Control of Oncofetal Gene Expression in Cancer. Molecular and Cellular Biology, pp.352-369

H. Zhang, H. Wu, J. Zheng, P. Yu, L. Xu et al., Transforming growth factor ?1 signal is crucial for dedifferentiation of cancer cells to cancer stem cells in osteosarcoma, Stem Cells, vol.31, issue.3, pp.433-446, 2013.

T. S. Zhu, M. A. Costello, C. E. Talsma, C. G. Flack, G. Jessica et al., , vol.71, pp.6061-6072, 2012.

Y. Zou, Y. Huang, J. Yang, J. Wu, and C. Luo, miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and selfrenewal capacity, Molecular Medicine Reports, vol.15, issue.4, pp.1631-1637, 2017.



, Liste des présentations des travaux de recherche Février 2015 : Présentation orale

, Super Besse, France " Calpain-6 expression identifies a stem cell population in osteosarcoma, Congrès des Journées Françaises des Tissus Minéralisés (JFBTM), 2015.

, Présentation Orale (10 min) PhD training course de l'European Calcified Tissue Society (ECTS), Oxford, Royaume-Unis "Calpain-6 acts as a cell fate determinant and identifies osteosarcoma stem cells, Prix reçu: New investigator award Juillet, 2016.

B. Journées, La calpaïne-6 identifie des cellules souche des ostéosarcomes, Groupe Sarcome Français), 2017.

J. Paris and D. , Calpaïne-6 : Biomarqueur des cellules souche des ostéosarcomes