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            <title xml:lang="en">Development of microscopy strategies for rapid imaging of zebrafish heart development</title>
            <title xml:lang="fr">Développement de stratégies de microscopie pour l'imagerie rapide du développement du cœur du poisson-zèbre</title>
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                <title xml:lang="en">Development of microscopy strategies for rapid imaging of zebrafish heart development</title>
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                <authority type="supervisor">Julien Vermot</authority>
                <authority type="supervisor">Florian Fahrbach</authority>
                <authority type="jury">Pascal Didier [Président]</authority>
                <authority type="jury">Willy Supatto [Rapporteur]</authority>
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                <term xml:lang="en">Light Sheet Fluorsescence Microscopy</term>
                <term xml:lang="en">Heart</term>
                <term xml:lang="en">Zebrafish</term>
                <term xml:lang="fr">Microscopie</term>
                <term xml:lang="fr">Light Sheet Fluorescence Microscopy</term>
                <term xml:lang="fr">Cœur</term>
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              <p>Structures inside the heart of zebrafish are highly dynamic, and span a broad range of and scales and velocities. The microscopy technique that best addresses the challenges posed by cardiac imaging is Light Sheet Fluorsescence Microscopy (LSFM). LSFM has only gained popularity since its introduction in biological research, which has led to the development of many techniques aiming at making the perfect light sheet. Yet, not a detailed and objective comparison between all the types of light sheets have been made. To fill this gap, we described a set of measures and compared the existing LSFM schemes. Our group is interested in the very dynamic processes in the heart. For that, we decided to implement a modular solution to attach to the Leica DLS Light Sheet system available in the institute that allows us to acquire images with high temporal resolution.</p>
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              <p>Les structures au cœur du poisson-zèbre sont très dynamiques et couvrent un large éventail des échelles et des vitesses. La technique de microscopie qui répond le mieux aux défis posés par l'imagerie de la morphogenese cardiaque est la microscopie en feuillet de luniere (Light Sheet Fluorescence Microscopy ou LSFM). LSFM a gagné en popularité, ce qui a genere un grand nombre d’approche et de montage optique permettant de créer des feuillets de lumiere avec des proprietes tres differentes. Pourtant, aucune comparaison détaillée et objective entre tous les types de feuilles de lumière ont été réalisés. Pour combler cette lacune, nous avons décrit un ensemble de mesures et avons comparé les different types de feuillet de lumieres existants. D’un otre cote, notre groupe s'intéresse aux processus très dynamiques du cœur. Nous avons developpe et mis en place une solution modulaire à attacher au Leica DLS Light Sheet disponible à l'institut qui nous permettrait d'acquérir des images avec une haute résolution temporelle.</p>
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