The habenula (Hb) is a key nucleus for monoamine control with circadian properties. However, its clockwork is not fully characterized. In this thesis, we confirm that the Hb harbors a molecular circadian clock which can be disrupted by mutations of clock genes. Furthermore, we showed that the Hb clock was still rhythmic even without the suprachiasmatic nucleus (SCN) or in constant light condition which disrupts locomotor activity rhythms and SCN oscillations. However, Hb oscillators (in the caudal and rostral part) are uncoupled in constant darkness condition. In the last part of the study, we determined the potential internal synchronizators. The results indicated that at least four molecules (dopamine, noradrenaline, vasopressin and orexin) affect the Hb circadian properties (period, amplitude). The characterization of the Hb clock will give us a better understanding of its involvement in psychiatric diseases combining rhythms and monoamine alteration like depression or addiction.