Marine infrastructures offer new substrates, colonized by a variety of organisms (biofouling), but are different from natural habitats since environmental filters and ecological processes differ, biotic composition differs from natural habitats, and they are characterized by a high diversity and abundance of non-indigenous species (NIS). Artificialization in marinas modifies hydrodynamism, affecting the water column characteristics like temperature and favoring the concentration of anthropic disturbances like pollutants. Environmental gradients may be present, with higher levels of disturbance in the innermost parts of marinas, acting as selective filters for organisms. The present work aimed to understand the diversity and functioning of sessile communities in marinas by studying how disturbance gradients shape community structure and function between the entrances of marinas and their more disturbed inner parts. A particular focus laid on NIS, which are common in marinas and constitute an important societal challenge. An experimental approach (reciprocal transplant) in six marinas from two regions (Mediterranean, Atlantic), revealed that differences in community structure are caused by local pollution levels, associated to local adaptation at small spatial scale (<100m). However, other processes might affect these dynamics like biotic interactions (predation) and pulse disturbances (heat waves). In the context of climate change, we also tested how projected seawater warming (+3°C) may impact marina communities and might favor NIS. These results might help understanding how disturbances in marine urban ecosystems drive biodiversity and especially the prevalence of NIS.