This PhD thesis is dedicated to the modeling and simulation of microscopic traffic behavior in virtual reality system, with the intent of providing a new approach to effectively ensure traffic safety. At first, Virtual Reality Intelligent Simulation System of Vehicles (VR-ISSV), based on multi-agent, is proposed to simulate the intelligent microscopic traffic, which is a hierarchical modular modeling and simulation system consisting of hardware, network and operating system layers, visualization management layer, multi-agent layer, human-machine interface layer. The multi-agent layer includes entity agents (intelligent vehicle agents and around vehicle agents), service agent and environment agent. Second, for the intelligent vehicle agent model, a decentralized design paradigm is used for developing the multi-controller based intelligent vehicle, whereby the car following behavior and the overtaking behavior could be realized by the coordination of the multi-controller. The environment agent is constructed based on the conception of Synthetic Natural Environment (SNE), taking into account the interaction between the vehicles and the natural environment. Geographic Information System (GIS) is used to establish environment agent. Finally, to ensure the safety in microscopic traffic maneuver, the intelligent vehicle controllers adapting to complex environment are considered. Fuzzy logic based controllers are designed for sending the appropriate outputs to the vehicle’s actuators – the steering wheel and the throttle/brake pedals. Microscopic traffic behavior models based on the intelligent vehicle agent involving environment are studied