Skip to Main content Skip to Navigation
New interface
Theses

Transmission hétérosexuelle de HIV : visualisation et modélisation de l'infection de la muqueuse génitale féminine

Abstract : Heterosexual transmission of HIV from men to women is the main route of contamination by this agent worldwide. This mode of transmission involves the contact between infected semen and healthy female genital mucosa, and then the crossing of cell-free or cell-associated viruses through the mucosa. The mechanisms involved in HIV transmission from men to women as well as the role of seminal plasma in selective transmission of R5-tropic viruses stay partially unknown. During the course of my PhD we visualized the crossing of cell-associated and cell-free virus through the endocervical genital mucosa. Initially, the study focused on the transmigration of infected immune cells through an in vitro model of endocervical mucosa, as well as on the role of seminal plasma in HIV transmission and selection of R5-tropic strains. Afterwards, we developed a fluorescent, replicative competent, infectious chimeric virus allowing its detection by confocal microscopy after several replication cycles. After contact of the endocervical mucosa with X4- or R5-tropic chimeric viruses, we visualized by confocal microscopy the infection of endocervical epithelial cells and the transmission of HIV to immune cells placed in the basal compartment. The use of a mathematical model allowed standardizing and quantifying the detection of infected cells in the mucosa. My PhD work describes as a first step the importance of the crossing of cell-associated HIV particles in the heterosexual transmission, as well as the involvement of seminal plasma in the selection of R5-tropic strains. Moreover, the virological model developed herein allows the direct visualization of HIV transmission through the female genital mucosa. I have thus demonstrated that it is possible to localize and quantify viral particles within the mucosa. This virological tool help to better understand the mechanisms involved in HIV heterosexual transmission and the selection of R5-tropic strains
Document type :
Theses
Complete list of metadata

Cited literature [250 references]  Display  Hide  Download

https://theses.hal.science/tel-00981483
Contributor : ABES STAR :  Contact
Submitted on : Tuesday, April 22, 2014 - 12:52:09 PM
Last modification on : Monday, June 21, 2021 - 4:05:17 PM
Long-term archiving on: : Monday, April 10, 2017 - 4:06:27 PM

File

These-Terrasse-Rachel-2012.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-00981483, version 1

Citation

Rachel Terrasse. Transmission hétérosexuelle de HIV : visualisation et modélisation de l'infection de la muqueuse génitale féminine. Médecine humaine et pathologie. Université Jean Monnet - Saint-Etienne, 2012. Français. ⟨NNT : 2012STET004T⟩. ⟨tel-00981483⟩

Share

Metrics

Record views

319

Files downloads

798