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Détection fluorimétrique en circuit microfluidique des ions Pb2+, Hg2+et Cd2+ en milieu aqueux

Abstract : This thesis is supported by a European project called "microfluiD" for the detection of organic pollutants by microfluidic devices (mycotoxins in animal feed, bacteria and heavy metals). Because of hazards Pb2+, Hg2+ and Cd2+ ions in the environment, it is important to increase the number of assays in tap water. The use of fluorescence or microlaser has many advantages: in addition to their low cost, sensitivity and selectivity, it is possible to design portable devices based on these systems in the field. Two approaches are mainly developed in this work: The first one is based on fluorescence. Two fluorescent sensors were synthesized: CalixDANS-3-OH for Pb2+ detection and DPPS-PEG for Hg2+ detection. Complexation of Hg2+and Pb2+ was at first performed in solution. The complexation of Cd2+ in microfluidic device from the commercial compound Rhod-5N is studied and very promising results of Hg2+ sensing by DPPS-PEG are obtained. We also studied the possibility to detect Pb2+ by CalixDANS-3-OH grafted onto the walls of the microfluidic device. Despite a degradation of the probe, we were able to detect low concentrations of Pb2+. A very good selectivity towards several interfering cations was obtained. The second approach is based on the use of microlasers. We synthesized two porous block polymers for Pb2+ and Hg2+ detection. Because of a bad solubility of the copolymers, we didn't succeed to fabricate microcavities from these polymers. A second strategy which consists in grafting the specific ligands of Pb2+ and Hg2+ on the PMMA microcavity is very promising. Proof of principle of the detection of Hg2+ is obtained by functionalizing mercaptopropyltriethoxysilane on the surface of PMMA. This work also led us to synthesize Bodipy laser dyes for the manufacturing of microcavities by two-photon polymerization (2PP).
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Contributor : Djibril Faye Connect in order to contact the contributor
Submitted on : Monday, August 6, 2012 - 1:51:46 PM
Last modification on : Wednesday, December 15, 2021 - 10:02:13 AM
Long-term archiving on: : Friday, December 16, 2016 - 5:13:48 AM


  • HAL Id : tel-00722906, version 1


Djibril Faye. Détection fluorimétrique en circuit microfluidique des ions Pb2+, Hg2+et Cd2+ en milieu aqueux. Chimie organique. École normale supérieure de Cachan - ENS Cachan, 2011. Français. ⟨NNT : ⟩. ⟨tel-00722906⟩



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