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Contribution à l'étude phytochimique d'orchidées tropicales : identification des constituants d'Aerides rosea et d'Acampe rigida : techniques analytiques et préparatives appliquées à Vanda coerulea et Vanda teres

Abstract : Phytochemical analyses of the composition of two orchids Aeridinae subtribe, Aerides rosea Lod . ex . Lindl . & Paxton and Acampe rigida (Buch. -Ham . Ex Sm.) P. F. Hunt were conducted using the state-of-the-art hyphenated techniques and a developed dereplication strategy. Ten phenanthrene derivatives have been identified in the stems of A. rosea, including two newly described structures. Four stilbenoids, three phenolic acids derivatives and four cinnamic acid esters were identified in the stems of A. rigida. We also performed quantitative assays of chemical tracers in different samples of two representative species from the Vandeae tribe: Vanda teres (Roxb.) Lindl. and Vanda coerulea Griff. ex. Lindl., in the aim to highlight possible variations in their composition, depending on environmental factors and vegetative growth stage. Finally, we have developed conditions for preparative isolation using centrifugal partition chromatography to purify biomarkers previously identified in the stems of Vanda teres: three glucosyloxybenzyl malates derivatives and their biosynthetic precursor.
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Submitted on : Wednesday, May 28, 2014 - 10:52:11 AM
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Veronika Čáková. Contribution à l'étude phytochimique d'orchidées tropicales : identification des constituants d'Aerides rosea et d'Acampe rigida : techniques analytiques et préparatives appliquées à Vanda coerulea et Vanda teres. Biologie végétale. Université de Strasbourg, 2013. Français. ⟨NNT : 2013STRAF035⟩. ⟨tel-00997451⟩

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