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Design and evaluation of a new diagnostic instrument for osmotic gradient ektacytometrie

Abstract : The ability of red blood cells (RBC) to change their shape under varying conditions is a crucial property allowing these cells to go through capillaries narrower than their own diameter. Ektacytometry is a technique for measuring deformability by exposing a highly diluted blood sample to shear stress and evaluating the resulting elongation in RBC shape using a laser diffraction pattern. This work contributes to the design and evaluation of a new diagnostic technique based on osmotic scan ektacytometry, using a microfluidic method. It allows the measurement of deformability of an RBC population, as a function of varying medium osmolality. This measurement makes possible a differential diagnosis for any one of a number of RBC disorders presenting similar symptoms. It also permits the physician to follow the effects of treatments. Both theoretical aspects based on flow equations and a proof of principle are discussed. This new technique opens up the possibility of building a simple, small footprint instrument described in this work that can be used with finger prick amounts of blood
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Submitted on : Tuesday, April 10, 2018 - 5:40:07 PM
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  • HAL Id : tel-01763128, version 1

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Arie Eric Oren Finkelstein. Design and evaluation of a new diagnostic instrument for osmotic gradient ektacytometrie. Electronics. Université Paris-Est, 2017. English. ⟨NNT : 2017PESC1163⟩. ⟨tel-01763128⟩

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